Anti-Androgen Receptor antibody [SP107] - N-terminal
- RabMAb
- Lab Essentials
- 20ul selling size
- What is this?
4
(8 Reviews)
|
(9 Publications)
Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) is a rabbit monoclonal antibody detecting Androgen Receptor in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human placenta tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human cervix tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate cancer tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human uterus tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human breast tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human testis tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Androgen Receptor with ab105225 at 1/400 dilution in immunohistochemical analysis.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Androgen Receptor with Purified ab105225 at 1/400 dilution (0.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 30mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- WB
Lab
Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Blocking/Diluting buffer and concentration : 5% NFDM /TBST
All lanes:
Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1.55 µg
Lane 1:
LnCAP (Human prostate carcinoma epithelial cell) cell lysate at 20 µg
Lane 2:
DU 145 (Human prostate carcinoma epithelial cell) cell lysate at 20 µg
Lane 3:
Mouse testis tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL
Predicted band size: 99 kDa
Observed band size: 120 kDa
false
- WB
Lab
Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
ab181602 was used as a GAPDH loading control.
All lanes:
Western blot - Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) at 1/1000 dilution
Lane 1:
Mouse liver tissue lysate at 20 µg
Lane 2:
Rat liver tissue lysate at 20 µg
Lane 3:
Mouse testis tissue lysate at 20 µg
Lane 4:
Rat testis tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
false
Exposure time: 60s
Related conjugates and formulations (1)
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Anti-Androgen Receptor antibody [SP107] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-Androgen Receptor antibody [SP107] - N-terminal (ab105225) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of Androgen Receptor?
Anti-Androgen Receptor [SP107] - N-terminal (ab105225) specifically detects a band for Androgen Receptor (UniProt: P10275) at a molecular weight of 99kDa.
Collaboration
Anti-Androgen Receptor [SP107] - N-terminal (ab105225) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Reviewed by scientists
Anti-Androgen Receptor [SP107] - N-terminal (ab105225) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [SP107] also available for your convenience: ab105225, Carrier free - ab236225, ab279422
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.
Pathways
AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.
Product protocols
- Visit the General protocols
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Target data
Publications (9)
Recent publications for all applications. Explore the full list and refine your search
Antioxidants (Basel, Switzerland) 13: PubMed39594482
2024
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Communications biology 7:740 PubMed38890503
2024
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Endocrinology 165: PubMed38788194
2024
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Cancer medicine 12:10816-10828 PubMed36951594
2023
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Small methods 7:e2201293 PubMed36538748
2022
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iScience 24:102303 PubMed33870126
2021
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Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 33:1056-1064 PubMed31896809
2020
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Oncotarget 9:35327-35342 PubMed30450161
2018
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European archives of oto-rhino-laryngology : offic 275:1885-1892 PubMed29785551
2018
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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