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Rabbit Recombinant Monoclonal Androgen Receptor antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Target data

Function

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.Isoform 3Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.Isoform 4Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Alternative names

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Rabbit Recombinant Monoclonal Androgen Receptor antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR15656-290

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

The AR-V7 is significant in cellular proliferation and survival processes without needing direct androgen binding due to its interaction capability with specific nuclear elements. It resides in the cell nucleus and engages with DNA as part of a transcriptional regulatory complex. Its ability to modulate the transcription of target genes makes it a prominent player in the regulation of cell cycle and apoptosis. In many instances AR-V7 forms complexes with other transcriptional modulators and nuclear proteins that augment its transcriptional activity.

Mechanical summary

The Androgen Receptor (AR-V7 specific) is a variant of the androgen receptor a type of nuclear receptor critical for mediating the effects of androgens like testosterone. AR-V7 is a protein that lacks the ligand-binding domain present in full-length androgen receptors resulting in its activity being independent of androgen presence. It is prominently expressed in prostate tissues and is often observed in prostate cancer cells resistant to conventional therapies. The protein variant exhibits a molecular mass of about 75 kDa. Commonly recognized aliases for AR-V7 include AR-V7 ARv7 and AR V7.

Pathway

AR-V7 plays an important role in the androgen signaling pathway which is pivotal in the progression and development of prostate cancer. It interacts with various co-regulators and influences the expression of genes involved in cell growth and differentiation. The splice variant also participates in other related signaling pathways such as the PI3K/AKT pathway indirectly interfacing with proteins like AR peptides and AR protein which further highlights its relevance in intracellular signaling mechanisms.

Disease

AR-V7 has a well-established connection with castration-resistant prostate cancer (CRPC) a form of prostate cancer that progresses despite androgen deprivation therapy. The presence of AR-V7 signifies a poor prognosis and resistance to anti-androgen therapies. Its transcriptional activity is linked to proteins such as AR protein and anti-androgen agents that typically target the full-length androgen receptor. Understanding the role of AR-V7 in reinforcing cancer cell survival and therapy resistance has fueled research towards developing targeted therapeutics and diagnostics like AR-V7 ELISA kits and AR IHC assays.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:PMID: 30334814).

    Negative control: LNCaP and PC-3 (PMID:27117751, PMID: 30334814).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500) at 1/1000 dilution

    Lane 1: 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 68 kDa

    Observed band size: 80 kDa

  • Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysate was made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 81 seconds.

    All lanes: Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500) at 1/1000 dilution

    All lanes: 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 80 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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