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AB273500

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

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(2 Reviews)

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(4 Publications)

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) is a rabbit monoclonal antibody detecting Androgen Receptor (AR-V7 specific) in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)
  • WB

Lab

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : PMID : 30334814).

Negative control : LNCaP and PC-3 (PMID : 27117751, PMID : 30334814).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 68 kDa

Observed band size: 80 kDa

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Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)
  • WB

Lab

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysate was made freshly and used in WB immediately to minimize protein degradation.

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution

All lanes:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 80 kDa

false

  • Carrier free

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free

  • 660 APC

    APC Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

  • 578 PE

    PE Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15656-290

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Androgen Receptor (AR-V7 specific)?
Anti-Androgen Receptor (AR-V7 specific) [EPR15656-290] (ab273500) specifically detects a band for Androgen Receptor (AR-V7 specific) (UniProt: P10275) at a molecular weight of 99kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR15656-290] also available for your convenience: ab273500, Carrier free - ab273511, PE - ab318410, APC - ab318513, Alexa Fluor® 488 - ab318616, Alexa Fluor® 647 - ab318719, Alexa Fluor® 594 - ab318822, Alexa Fluor® 555 - ab318922

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Androgen Receptor (AR-V7 specific) is a variant of the androgen receptor a type of nuclear receptor critical for mediating the effects of androgens like testosterone. AR-V7 is a protein that lacks the ligand-binding domain present in full-length androgen receptors resulting in its activity being independent of androgen presence. It is prominently expressed in prostate tissues and is often observed in prostate cancer cells resistant to conventional therapies. The protein variant exhibits a molecular mass of about 75 kDa. Commonly recognized aliases for AR-V7 include AR-V7 ARv7 and AR V7.
Biological function summary

The AR-V7 is significant in cellular proliferation and survival processes without needing direct androgen binding due to its interaction capability with specific nuclear elements. It resides in the cell nucleus and engages with DNA as part of a transcriptional regulatory complex. Its ability to modulate the transcription of target genes makes it a prominent player in the regulation of cell cycle and apoptosis. In many instances AR-V7 forms complexes with other transcriptional modulators and nuclear proteins that augment its transcriptional activity.

Pathways

AR-V7 plays an important role in the androgen signaling pathway which is pivotal in the progression and development of prostate cancer. It interacts with various co-regulators and influences the expression of genes involved in cell growth and differentiation. The splice variant also participates in other related signaling pathways such as the PI3K/AKT pathway indirectly interfacing with proteins like AR peptides and AR protein which further highlights its relevance in intracellular signaling mechanisms.

AR-V7 has a well-established connection with castration-resistant prostate cancer (CRPC) a form of prostate cancer that progresses despite androgen deprivation therapy. The presence of AR-V7 signifies a poor prognosis and resistance to anti-androgen therapies. Its transcriptional activity is linked to proteins such as AR protein and anti-androgen agents that typically target the full-length androgen receptor. Understanding the role of AR-V7 in reinforcing cancer cell survival and therapy resistance has fueled research towards developing targeted therapeutics and diagnostics like AR-V7 ELISA kits and AR IHC assays.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed : 19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.. Isoform 3. Lacks the C-terminal ligand-binding domain and therefore constitutively regulates the transcription of a specific set of canonical AR-target genes, including PSA/KLK3 and TMPRSS2, independently of steroid hormones (PubMed : 19244107, PubMed : 25008967). However, some genes are differentially regulated by full-length AR (isoform 1) and isoform 3. Isoform 3-specific target genes may be regulated independently of FOXA1 expression (PubMed : 25008967).. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
See full target information AR
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70001 PubMed39558820

2024

Extracellular vesicles containing SARS-CoV-2 proteins are associated with multi-organ dysfunction and worse outcomes in patients with severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Diego de Miguel-Perez,Marisol Arroyo-Hernandez,Sabrina La Salvia,Muthukumar Gunasekaran,Edward M Pickering,Stephanie Avila,Etse Gebru,Eduardo Becerril-Vargas,Sergio Monraz-Perez,Kapil Saharia,Alison Grazioli,Michael T McCurdy,Matthew Frieman,Lisa Miorin,Alessandro Russo,Andrés F Cardona,Adolfo García-Sastre,Sunjay Kaushal,Fred R Hirsch,Djordje Atanackovic,Susmita Sahoo,Oscar Arrieta,Christian Rolfo

International journal of molecular sciences 25: PubMed39518967

2024

2-Bromopalmitate-Induced Intestinal Flora Changes and Testicular Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Ma,Yining Chen,Long Li,Zifang Wu,Heran Cao,Chao Zhu,Qimin Liu,Yang Wang,Shaoxian Chen,Yuyan Liu,Wuzi Dong

Journal of leukocyte biology 111:19-32 PubMed34448502

2021

Adipose-derived stem cell-derived extracellular vesicles inhibit neuroblastoma growth by regulating GABBR1 activity through LINC00622-mediated transcription factor AR.

Applications

Unspecified application

Species

Unspecified reactive species

Mengguo Guo,Dongpeng Li,Yawen Feng,Mu Li,Bo Yang

Antioxidants (Basel, Switzerland) 10: PubMed34209652

2021

Melatonin Ameliorates the Toxicity Induced by Deoxynivalenol in Murine Ovary Granulosa Cells by Antioxidative and Anti-Inflammatory Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Hairui Fan,Shiqin Wang,Haifei Wang,Mingan Sun,Shenglong Wu,Wenbin Bao
View all publications
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Product promise

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