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Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) is a rabbit monoclonal antibody that is used to detect Androgen Receptor (AR-V7 specific) in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500), expandable thumbnail
  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500), expandable thumbnail
  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (AB273500), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Target data

Function

The protein expressed by the AR gene functions as a steroid hormone receptor, a type of ligand-activated transcription factor that regulates eukaryotic gene expression and influences cellular proliferation and differentiation in target tissues. Its transcription factor activity can be modulated by coactivator and corepressor proteins, such as ZBTB7A, which recruits NCOR1 and NCOR2 to androgen response elements on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. The transcription activation can also be down-regulated by NR0B2. The protein is activated, though not phosphorylated, by HIPK3 and ZIPK/DAPK3. Notably, isoforms 3 and 4 of this protein lack the C-terminal ligand-binding domain and may thus constitutively activate transcription of specific gene sets independently of steroid hormones. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Recommended products

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) is a rabbit monoclonal antibody that is used to detect Androgen Receptor (AR-V7 specific) in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15656-290
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.

What is the molecular weight of Androgen Receptor (AR-V7 specific)?


Anti-Androgen Receptor (AR-V7 specific) [EPR15656-290] (ab273500) specifically detects a band for Androgen Receptor (AR-V7 specific) (UniProt: P10275) at a molecular weight of 99kDa.

Recommended positive controls


WB: 22Rv1 whole cell lysate. ICC/IF: 22Rv1 cells. Flow Cyt (intra): 22Rv1 cells.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR15656-290] also available for your convenience:
ab273500, Carrier free - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free ab273511, PE - PE Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318410, APC - APC Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318513, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318616, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318719, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318822, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] ab318922



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Androgen Receptor (AR-V7 specific) is a variant of the androgen receptor a type of nuclear receptor critical for mediating the effects of androgens like testosterone. AR-V7 is a protein that lacks the ligand-binding domain present in full-length androgen receptors resulting in its activity being independent of androgen presence. It is prominently expressed in prostate tissues and is often observed in prostate cancer cells resistant to conventional therapies. The protein variant exhibits a molecular mass of about 75 kDa. Commonly recognized aliases for AR-V7 include AR-V7 ARv7 and AR V7.

Biological function summary

The AR-V7 is significant in cellular proliferation and survival processes without needing direct androgen binding due to its interaction capability with specific nuclear elements. It resides in the cell nucleus and engages with DNA as part of a transcriptional regulatory complex. Its ability to modulate the transcription of target genes makes it a prominent player in the regulation of cell cycle and apoptosis. In many instances AR-V7 forms complexes with other transcriptional modulators and nuclear proteins that augment its transcriptional activity.

Pathways

AR-V7 plays an important role in the androgen signaling pathway which is pivotal in the progression and development of prostate cancer. It interacts with various co-regulators and influences the expression of genes involved in cell growth and differentiation. The splice variant also participates in other related signaling pathways such as the PI3K/AKT pathway indirectly interfacing with proteins like AR peptides and AR protein which further highlights its relevance in intracellular signaling mechanisms.

Associated diseases and disorders

AR-V7 has a well-established connection with castration-resistant prostate cancer (CRPC) a form of prostate cancer that progresses despite androgen deprivation therapy. The presence of AR-V7 signifies a poor prognosis and resistance to anti-androgen therapies. Its transcriptional activity is linked to proteins such as AR protein and anti-androgen agents that typically target the full-length androgen receptor. Understanding the role of AR-V7 in reinforcing cancer cell survival and therapy resistance has fueled research towards developing targeted therapeutics and diagnostics like AR-V7 ELISA kits and AR IHC assays.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:PMID: 30334814).

    Negative control: LNCaP and PC-3 (PMID:27117751, PMID: 30334814).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution

    Lane 1: 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 68 kDa

    Observed band size: 80 kDa

  • Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500), expandable thumbnail

    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysate was made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 81 seconds.

    All lanes: Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] (ab273500) at 1/1000 dilution

    All lanes: 22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 80 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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