Rabbit Recombinant Monoclonal Androgen Receptor antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human AR.
Images
![Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--western-blot-img407910.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335)")
![Immunohistochemistry paraffin embedded sections - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--immunohistochemistry-paraffin-embedded-sections-img407909.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335)")
![Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--immunocytochemistry-immunofluorescence-img407911.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (AB308335)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human AR. The exact immunogen used to generate this antibody is proprietary information. Database link P10275
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Alternative names
DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
Recommended products
Rabbit Recombinant Monoclonal Androgen Receptor antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human AR.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human AR. The exact immunogen used to generate this antibody is proprietary information. Database link P10275
- Carrier free
- Yes
- Clone number
- HL1028
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
- Biological function summary
AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.
- Pathways
AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.
- Associated diseases and disorders
AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--western-blot-img407910.jpg "Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)")
Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (ab308335) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335) at 1/1000 dilution
Lane 1: 22RV1 cell lysate at 30 µg
Lane 2: LNCap cell lysate at 30 µg
Lane 3: MCF7 cell lysate at 30 µg
Lane 4: DU145 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 99.2 kDa
![Immunohistochemistry paraffin embedded sections - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--immunohistochemistry-paraffin-embedded-sections-img407909.jpg "Immunohistochemistry paraffin embedded sections - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)")
Immunohistochemistry paraffin embedded sections - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)
Immunohistochemical analysis of mouse testis tissue labeling Androgen Receptor ARv7 Splice Variant using ab308335 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335), expandable thumbnail](https://content.abcam.com/products/images/androgen-receptor-arv7-splice-variant-antibody-hl1028-ab308335--immunocytochemistry-immunofluorescence-img407911.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)")
Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor ARv7 Splice Variant antibody [HL1028] (ab308335)
For immunofluorescence analysis, 22RV1 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes for detection of Androgen Receptor (ARv7 Splice Variant) using ab308335 at 1/500 dilution (green). Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000.
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