Rabbit Polyclonal Angiogenin antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ANG.
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- BMC neuroscience 21:242020Dl-3-n-Butylphthalide promotes neovascularization and neurological recovery in a rat model of intracerebral hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwen Tu et. al.PubMed 32471341
- Biomedicine & pharmacotherapy = Biomedecine & phar 105:566-5722018ZNF750 inhibited the malignant progression of oral squamous cell carcinoma by regulating tumor vascular microenvironment.
- Molecular medicine reports 17:7866-78742018Puerarin regulates neovascular glaucoma through pigment epithelium‑derived growth factor‑induced NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Yu Wei et. al.PubMed 29620183
- American journal of physiology. Lung cellular and 306:L1104-162014Dynamic expression of chymotrypsin-like elastase 1 over the course of murine lung development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Liu et. al.PubMed 24793170
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANG. The exact immunogen used to generate this antibody is proprietary information. Database link P03950
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Predicted |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed:12051708, PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:23843625, PubMed:2424496, PubMed:2459697, PubMed:2730651, PubMed:27518564, PubMed:28176817, PubMed:29100074, PubMed:29748193, PubMed:3122207, PubMed:32510170, PubMed:38718836, PubMed:8159680, PubMed:8570639, PubMed:8622921, PubMed:9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed:29100074, PubMed:32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed:1400510, PubMed:19332886, PubMed:20129916, PubMed:21855800, PubMed:23047679, PubMed:27518564, PubMed:29100074, PubMed:29748193, PubMed:32510170, PubMed:38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed:3289612, PubMed:38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed:31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed:12051708, PubMed:15735021, PubMed:27518564, PubMed:29100074, PubMed:8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed:19354288, PubMed:4074709, PubMed:8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed:27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed:32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).
Alternative names
RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5
Recommended products
Rabbit Polyclonal Angiogenin antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ANG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANG. The exact immunogen used to generate this antibody is proprietary information. Database link P03950
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiogenin also known as ANG is a small protein with a molecular mass of approximately 14 kDa. It belongs to the ribonuclease A superfamily. Angiogenin expression occurs in a variety of tissues with notably pronounced levels in the liver pancreas and placenta indicating its widespread biological importance. In addition to its primary name it appears in the context of research under alternative names like KIT ANG and elisa ang reflecting its use and identification in various experimental assays.
- Biological function summary
Angiogenin significantly contributes to the process of angiogenesis the formation of new blood vessels from pre-existing vascular structures. It characterizes acts as both a ribonuclease and a potent angiogenic factor. Although not part of a multi-protein complex angiogenin interacts with endothelial cells to promote their growth and migration. Angiogenin facilitates these cellular behaviors by triggering pathways that enhance cell survival and proliferation essential for healthy tissue development and repair.
- Pathways
Angiogenin is intricately involved in the angiogenesis pathway a critical process for both physiological and pathological conditions. It interacts with proteins such as VEGF (vascular endothelial growth factor) which plays an essential role in vascular function. Angiogenin's interaction with these pathways enhances cellular responses to external stimuli maintaining vascular integrity and supporting tissue regeneration under stress or injury conditions.
- Associated diseases and disorders
Angiogenin has connections to conditions like cancer and amyotrophic lateral sclerosis (ALS). Aberrant regulation of angiogenin can contribute to tumorigenesis by promoting excessive angiogenesis aiding the growth and spread of cancerous cells. In ALS altered angiogenin function associates with neurodegenerative processes linking its function to proteins such as superoxide dismutase 1 (SOD1). Understanding angiogenin's role in these diseases aids in uncovering therapeutic targets and developing treatment strategies.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Angiogenin antibody (ab139947)
Lane 1: Western blot - Anti-Angiogenin antibody (ab139947) at 1 µg/mL
Lane 2: Western blot - Anti-Angiogenin antibody (ab139947) at 2 µg/mL
All lanes: Rat liver tissue lysate at 15 µg
Predicted band size: 17 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Angiogenin antibody (ab139947)
Angiogenin western blot using anti-Angiogenin antibody ab139947. Publication image and figure legend from Tu, E., Chen, Q., et al., 2020, BMC Neurosci, PubMed 32471341.
ab139947 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab139947 please see the product overview.
Effect of NBP treatment on the expression of the vascular endothelial growth factor (VEGF) and angiopoietins-2 (Ang-2) proteins in tissue lysates of the injection site in a rat model of ICH. Panel (a) shows representative immunoblot images (upper two sets) of VEGF and Ang-2 in the groups as indicated, with representative images (bottom set) showing equal protein loading as blotted with the β-actin antibody. Panels (b, c) plot the protein levels expressed as relative optic densities (o.d.) normalized to the internal control β-actin. Results from two-way ANOVA followed by Bonferroni’s post hoc tests are marked with symbols, with * indicating significantly different comparing to the Sham groups; # indicating significantly different comparing to the ICH groups; and & indicating significantly different comparing to the ICH + NBP10 groups
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