Anti-Angiogenin antibody [EPR29570-541]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Immunohistochemical analysis of paraffin-embedded Human hepatic carcinoma tissue labeling Angiogenin with ab324526 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human hepatic carcinoma (PMID 25567797). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Angiogenin with ab324526 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon (PMID : 32510170). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Angiogenin with ab324526 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on human spleen. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Immunohistochemical analysis of paraffin-embedded (A) Untreated HepG2 (human hepatocellular carcinoma epithelial cell) cell pellet, (B) HepG2 treated with 300 ng/ml BFA for 24 hours cell pellet, (C) Untreated Daudi (human Burkitt's lymphoma lymphoblast) cell pellet and (D) Daudi treated with 300 ng/ml BFA for 24 hours cell pellet labeling Angiogenin with ab324526 at 1/1000 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Weak positive on (A) Untreated HepG2 cell pellet, positive staining on (B) HepG2 treated with 300 ng/ml BFA for 24 hours cell pellet, no staining on (C) Untreated Daudi cell pellet, and (D) Daudi treated with 300 ng/ml BFA for 24 hours cell pellet. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Angiogenin with ab324526 at 1/500 (0.99 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Daudi, Ramos

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29570-541] (ab324526) at 1/1000 dilution

Lane 1:

Untreated HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Lane 3:

Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A549 treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Lane 5:

Untreated Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 6:

Daudi treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Lane 7:

Untreated Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 8:

Ramos treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 14 kDa,36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, cerebellum

The identity of the higher MW band at approximately 100 kDa (in lane 3 and lane 4) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29570-541] (ab324526) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human cerebellum tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 14 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Angiogenin antibody [EPR29570-541] (AB324526)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse Angiopoietin 2, Angiopoietin 3 and Angiopoietin 4 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29570-541] (ab324526) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Angiogenin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse Angiopoietin 2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse Angiopoietin 3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse Angiopoietin 4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 14 kDa,36 kDa

false

Exposure time: 3s