Anti-Angiogenin antibody [EPR29571-709]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Angiogenin with ab326337 at 1/1000 (0.492 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : weak staining on rat spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Angiogenin with ab326337 at 1/1000 (0.492 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat colon.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Angiogenin with ab326337 at 1/1000 (0.492 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum, spleen.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29571-709] (ab326337) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 50 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Myc tag® antibody (1 : 5000) (15-30KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29571-709] (ab326337) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse ANG expression vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse ANG2 expression vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse ANG3 expression vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse ANG4 expression vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse ANG5 expression vector containing a Myc-tag, whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a mouse ANG6 expression vector containing a Myc-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa,15-30 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Angiogenin antibody [EPR29571-709] (AB326337)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum, spleen.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Angiogenin antibody [EPR29571-709] (ab326337) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 50 µg

Lane 2:

Mouse brain tissue lysate at 50 µg

Lane 3:

Mouse breast tissue lysate at 50 µg

Lane 4:

Mouse cerebellum tissue lysate at 50 µg

Lane 5:

Mouse spleen tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 180s