AB244534

Anti-Angiomotin antibody

Name: Anti-Angiomotin antibody (ab244534) | Abcam
Brand: Abcam Limited
SKU: AB244534
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Angiomotin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AMOT aa 200-350.

View Alternative Names

KIAA1071, AMOT, Angiomotin

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Angiomotin antibody (AB244534)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Angiomotin antibody (AB244534)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Angiomotin (green) using ab244534 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AMOT aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q4VCS5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiomotin also known as AMOT is a protein that has significant roles in various cellular processes. It has a molecular mass of approximately 130 kDa. Angiomotin is widely expressed across several tissues including endothelial cells where it functions as a regulator of cell migration and angiogenesis. It interacts directly with actin filaments affecting cell shape and movement therefore playing a role in the structural organization of cells.

Biological function summary

Angiomotin influences endothelial cell behavior through its involvement in cellular proliferation and mobility. Angiomotin forms part of a complex network by binding with other proteins such as Patj and LATS1/2 aiding in the control of the Hippo signaling pathway. This activity regulates organ size and cellular growth impacting tissue architecture and repair mechanisms in the body.

Pathways

Angiomotin's interactions contribute notably to the Hippo and MAPK signaling pathways. Within the Hippo pathway it aid the suppression of excessive cell proliferation by keeping the activity of YAP and TAZ in check. In the context of the MAPK pathway Angiomotin regulates signals that influence cell division and differentiation. The protein influences cellular outcomes by cooperating with other proteins like YAP a transcriptional coactivator often implicated in organ size and cancer.

Angiomotin is associated with various vascular conditions and cancers. Altered expression or dysregulation can contribute to pathological angiogenesis which plays a role in tumor progression and metastasis. In cancer Angiomotin impacts the Hippo pathway's ability to control YAP's oncogenic potential affecting cellular proliferation and tumor development. Understanding these interactions offers potential therapeutic targets for disorders linked to abnormal cell growth and vascular anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions (PubMed : 21205866).

See full target information AMOT

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1618012 PubMed40821844

2025

CRIF1 gene therapy ameliorates inflammatory bowel disease by suppressing TH17 cells and fibrosis through mitochondrial function regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Sil Park,Hye Yeon Kang,Ha Yeon Jeong,SeungCheon Yang,JeongWon Choi,Sang Hee Cho,Sol Kim,Bo-In Lee,Mi-La Cho

EMBO reports 25:1886-1908 PubMed38413734

2024

A neuroligin-2-YAP axis regulates progression of pancreatic intraepithelial neoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Middonti,Elena Astanina,Edoardo Vallariello,Roxana Maria Hoza,Jasna Metovic,Rosella Spadi,Carmen Cristiano,Mauro Papotti,Paola Allavena,Francesco Novelli,Sushant Parab,Paola Cappello,Aldo Scarpa,Rita Lawlor,Massimo Di Maio,Marco Arese,Federico Bussolino

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Angiomotin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Angiomotin antibody (AB244534)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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