Rabbit Recombinant Monoclonal Angiomotin like 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] - BSA and Azide free (AB249688), expandable thumbnail](https://content.abcam.com/products/images/angiomotin-like-1-antibody-epr11803-97-bsa-and-azide-free-ab249688--western-blot-img64390.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] - BSA and Azide free (AB249688)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.
Alternative names
Angiomotin-like protein 1, AMOTL1
Recommended products
Rabbit Recombinant Monoclonal Angiomotin like 1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11803-97
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249688 is the carrier-free version of Anti-Angiomotin like 1 antibody [EPR11803-97] ab171976.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiomotin like 1 (AMOTL1) also known as Angiomotin-like protein 1 is a member of the angiomotin family known for its role in angiogenesis. This protein has a molecular mass of approximately 90 kDa and is expressed in various tissues including endothelial cells. AMOTL1 interacts with other proteins to influence cell shape and migration by maintaining the integrity of tight junctions and actin cytoskeleton organization.
- Biological function summary
Angiomotin-like protein 1 plays a role in regulating cell proliferation and migration. AMOTL1 associates with the angiomotin protein complex which includes angiomotin and angiomotin like 2 (AMOTL2). The protein has functions in signaling pathways that are important for tissue architecture and cellular polarity. AMOTL1 facilitates the localization and stabilization of other cell signaling proteins to coordinate these complex biological processes effectively.
- Pathways
Angiomotin like protein 1 is involved in the Hippo signaling pathway a critical regulator of cell growth and apoptosis. In this pathway AMOTL1 interacts with proteins such as YAP/TAZ acting as a spatial regulator that limits their activity within the nucleus. Another important pathway involving AMOTL1 is the tight junction pathway where it helps maintain cell-cell adhesion and tissue integrity by interacting with ZO-1 and other structural proteins.
- Associated diseases and disorders
Angiomotin-like protein 1 has been linked to cancer progression particularly in breast and liver cancers. AMOTL1 influences tumor cell migration and invasion by modulating the localization of YAP/TAZ proteins. Additionally studies have shown a relation to vascular diseases as AMOTL1 affects endothelial cell function and angiogenesis potentially connecting it to disorders like atherosclerosis. Understanding these relationships may aid in developing targeted therapies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] - BSA and Azide free (ab249688), expandable thumbnail](https://content.abcam.com/products/images/angiomotin-like-1-antibody-epr11803-97-bsa-and-azide-free-ab249688--western-blot-img64390.jpg "Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] - BSA and Azide free (ab249688)")
Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] - BSA and Azide free (ab249688)
This data was developed using Anti-Angiomotin like 1 antibody [EPR11803-97] ab171976, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Angiomotin like 1 antibody [EPR11803-97] (Anti-Angiomotin like 1 antibody [EPR11803-97] ab171976) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Predicted band size: 107 kDa
Downloads
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