Rabbit Polyclonal Angiopoietin 1 antibody. Suitable for WB, IHC-P, ELISA, ICC/IF and reacts with Human, Mouse, Pig samples. Cited in 56 publications. Immunogen corresponding to Synthetic Peptide within Mouse Angpt1 aa 1-50.
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- Molecules (Basel, Switzerland) 28:2023Utilizing Contrast-Enhanced Ultrasonography with Phosphatidylserine Microbubbles to Detect Placental Inflammation in Rhesus Macaques.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel C Wilson,Jamie O Lo,Gabriel Romero Jimenez,Jonathan R Lindner,Ov D Slayden,Victoria H J RobertsPubMed 37049657
- PeerJ 11:e145622023Effects of swimming before and during pregnancy on placental angiogenesis and perinatal outcome in high-fat diet-fed mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofeng Zhu,Weiwei Chen,Haitang WangPubMed 36846460
- Frontiers in molecular biosciences 9:10501122022Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Smeda,Agnieszka Jasztal,Ebrahim H Maleki,Anna Bar,Magdalena Sternak,Grzegorz Kwiatkowski,Joanna Suraj-Prażmowska,Bartosz Proniewski,Anna Kieronska-Rudek,Kamila Wojnar-Lason,Klaudia Skrzypek,Marcin Majka,Karolina Chrabaszcz,Kamilla Malek,Stefan ChlopickiPubMed 36504711
- Journal of cell communication and signaling 17:609-6262022Quinacrine and Curcumin in combination decreased the breast cancer angiogenesis by modulating ABCG2 via VEGF A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepika Nayak,Subarno Paul,Chinmay Das,Subhasmita Bhal,Chanakya Nath KunduPubMed 36326988
- Frontiers in medicine 9:8692842022Intrarenal Single-Cell Sequencing of Hepatitis B Virus Associated Membranous Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeilin Yu,Wei Lin,Chanjuan Shen,Ting Meng,Peng Jin,Xiang Ding,Peter J Eggenhuizen,Joshua D Ooi,Rong Tang,Wannian Nie,Xia Li,Xiangcheng Xiao,Yong ZhongPubMed 35935760
- Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 37:1844-18562022Microvascular remodeling and altered angiogenic signaling in human kidneys distal to occlusive atherosclerotic renal artery stenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNattawat Klomjit,Xiang-Yang Zhu,Alfonso Eirin,Aditya S Pawar,Sabena M Conley,Amrutesh S Puranik,Christopher M Ferguson,Seo Rin Kim,Hui Tang,Kyra L Jordan,Ishran M Saadiq,Amir Lerman,Joseph P Grande,Stephen C Textor,Lilach O LermanPubMed 35451482
- Cell reports 37:1099962021Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeon-Ju Kim,Yi Zhao,Jae Kyung Myung,Joo Mi Yi,Min-Jung Kim,Su-Jae LeePubMed 34818544
- Cell reports 36:1095222021The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Luck,Andromachi Karakatsani,Bhavin Shah,Geza Schermann,Heike Adler,Janina Kupke,Nathalie Tisch,Hyun-Woo Jeong,Michaela Kerstin Back,Florian Hetsch,Anna D'Errico,Michele De Palma,Ellen Wiedtke,Dirk Grimm,Amparo Acker-Palmer,Jakob von Engelhardt,Ralf H Adams,Hellmut G Augustin,Carmen Ruiz de AlmodóvarPubMed 34407407
- Frontiers in molecular biosciences 8:6823672021The Inhibitory Effect of Lysophosphatidylcholine on Proangiogenesis of Human CD34 Cells Derived Endothelial Progenitor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijun Zhao,Yanhui HePubMed 34179086
- Molecules and cells 44:254-2662021Overexpression of COMP-Angiopoietin-1 in -Expressing Cells Impairs Hematopoiesis and Disturbs Erythrocyte Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Jaung Sim,Min-Hye Kim,Govinda Bhattarai,Jae-Won Hwang,Han-Sol So,Sher Bahadur Poudel,Eui-Sic Cho,Sung-Ho Kook,Jeong-Chae LeePubMed 33935045
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Angpt1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O08538
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Reactivity data
Select an application| WB | IHC-P | ELISA | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Tested | Expected | Expected | Expected |
| Pig | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein. |
Species Mouse | Dilution info 1/500 - 1/2000 | Notes Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein. |
Species Pig | Dilution info 1/500 - 1/2000 | Notes Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/800 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/25000 | Notes - |
Species Mouse | Dilution info 1/5000 - 1/25000 | Notes - |
Species Pig | Dilution info 1/5000 - 1/25000 | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Alternative names
KIAA0003, ANGPT1, Angiopoietin-1, ANG-1
Recommended products
Rabbit Polyclonal Angiopoietin 1 antibody. Suitable for WB, IHC-P, ELISA, ICC/IF and reacts with Human, Mouse, Pig samples. Cited in 56 publications. Immunogen corresponding to Synthetic Peptide within Mouse Angpt1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Angpt1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O08538
- Purity
- Whole antiserum
- Specificity
Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.
- Concentration
- Purification notes
his product was prepared from monospecific antiserum by a delipidation and defibrination.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, Anti-Angiopoietin 2/ANG2 antibody ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiopoietin 1 also known as Ang1 or Angpt1 is a protein important to the vascular system with a molecular mass of around 70 kDa. It is expressed widely in endothelial cells and fibroblasts. Angiopoietin 1 functions mechanically to bind the Tie2 receptor triggering signaling pathways that regulate blood vessel maturation and stability. This interaction is important for the development and maintenance of the blood vessel network.
- Biological function summary
Angiopoietin 1 supports the structural integrity of blood vessels by interacting with the Tie2 receptor as part of a signaling complex. It helps prevent vascular leak and promotes endothelial cell survival migration and adhesion. By playing such roles Angiopoietin 1 is fundamental for maintaining vascular homeostasis and facilitating proper blood flow throughout the body. It also influences the immune response by modulating endothelial functions.
- Pathways
Angiopoietin 1's involvement is most notable within the PI3K/AKT pathway and the MAPK pathway which are important for signaling cell survival and growth. These pathways work closely with other proteins such as VEGF (Vascular Endothelial Growth Factor) in angiogenesis and vascular remodeling. Angiopoietin 1 interacts with its related pathways to ensure that blood vessels remain functional and adaptive to physiological changes.
- Associated diseases and disorders
Angiopoietin 1 plays significant roles in cardiovascular diseases like atherosclerosis and diabetic vascular complications. Its dysregulation can lead to abnormalities in vascular maintenance and repair. There is also an association between Angiopoietin 1 and proteins like VEGF in cancer as both have roles in tumor angiogenesis. Understanding Angiopoietin 1's function could provide insights into therapeutic strategies for these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
This image is courtesy of Marion Scharpfenecker 2002Western blot - Anti-Angiopoietin 1 antibody (ab8451)
Supernatants of mouse-angiopoietin-expressing endothelial cells.
Lane 1 - wt endothelial cell
Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
Lane 4 - mouse Ang-2 (clone 2-9) expressing cellsAll lanes: Western blot - Anti-Angiopoietin 1 antibody (ab8451)
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab8451)
ab8451 was diluted 1:500 to detect Angiopoietin 1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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