AB250679

Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Angiopoietin 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA0003, ANGPT1, Angiopoietin-1, ANG-1

2 Images

Supplier Data

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (AB250679)

This data was developed using ab183701, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (<a href='/en-us/products/primary-antibodies/angiopoietin-1-antibody-epr2888n-ab183701'>ab183701</a>) at 1/50000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

ECV-304 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

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Lab

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (AB250679)

This data was developed using ab183701, the same antibody clone in a different buffer formulation.

This antibody is unsuitable to detect tissue lysate samples.

All lanes:

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (<a href='/en-us/products/primary-antibodies/angiopoietin-1-antibody-epr2888n-ab183701'>ab183701</a>) at 1/10000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 4:

Human brain lysates at 20 µg

Lane 5:

Mouse brain lysates at 20 µg

Lane 6:

Rat brain lysates at 20 µg

Lane 7:

Human heart lysates at 20 µg

Lane 8:

Mouse heart lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 57 kDa

Observed band size: 70 kDa

false

Unconjugated

Anti-Angiopoietin 1 antibody [EPR2888(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2888(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250679 is the carrier-free version of ab183701.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin 1 also known as Ang1 or Angpt1 is a protein important to the vascular system with a molecular mass of around 70 kDa. It is expressed widely in endothelial cells and fibroblasts. Angiopoietin 1 functions mechanically to bind the Tie2 receptor triggering signaling pathways that regulate blood vessel maturation and stability. This interaction is important for the development and maintenance of the blood vessel network.

Biological function summary

Angiopoietin 1 supports the structural integrity of blood vessels by interacting with the Tie2 receptor as part of a signaling complex. It helps prevent vascular leak and promotes endothelial cell survival migration and adhesion. By playing such roles Angiopoietin 1 is fundamental for maintaining vascular homeostasis and facilitating proper blood flow throughout the body. It also influences the immune response by modulating endothelial functions.

Pathways

Angiopoietin 1's involvement is most notable within the PI3K/AKT pathway and the MAPK pathway which are important for signaling cell survival and growth. These pathways work closely with other proteins such as VEGF (Vascular Endothelial Growth Factor) in angiogenesis and vascular remodeling. Angiopoietin 1 interacts with its related pathways to ensure that blood vessels remain functional and adaptive to physiological changes.

Angiopoietin 1 plays significant roles in cardiovascular diseases like atherosclerosis and diabetic vascular complications. Its dysregulation can lead to abnormalities in vascular maintenance and repair. There is also an association between Angiopoietin 1 and proteins like VEGF in cancer as both have roles in tumor angiogenesis. Understanding Angiopoietin 1's function could provide insights into therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

See full target information ANGPT1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (AB250679)

Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (AB250679)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise