AB276042

Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Carrier free. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANGPT2.

View Alternative Names

Angiopoietin-2, ANG-2, ANGPT2

3 Images

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Immunocytochemistry - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Immunofluorescence staining of Angiopoietin 2/ANG2 in A549 (Angiopoietin 2/ANG2) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276042 at 1/300 at 4 °C overnight. Then cells were stained with the AlexaFluor^® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

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Immunoprecipitation - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Angiopoietin 2/ANG2 was immunoprecipitated from the following whole cell lysates :

Lane A : 0.5 mg HepG2 (Angiopoietin 2/ANG2) whole Cell Lysate
Lane B : 0.5 mg A549 (Angiopoietin 2/ANG2) whole Cell Lysate
Lane C : 0.5 mg HEK-293T (Angiopoietin 2/ANG2) whole Cell Lysate

0.5 μL ab276042 and 15 μl of 50 % Protein G agarose.
Primary antibody : ab276042, at 1/500 dilution
Secondary antibody : Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

All lanes:

Immunoprecipitation - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)

Predicted band size: 56 kDa

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Western blot - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Performed under reducing conditions.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

A549 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

117

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANGPT2.

O15123

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "0.2-1 μL/mg of lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).

See full target information ANGPT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:585 PubMed35949323

2022

HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Shanshan Gao

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free

Immunocytochemistry - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Immunoprecipitation - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (AB276042)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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