Anti-Angiopoietin 2/ANG2 antibody
3
(4 Reviews)
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(42 Publications)
Rabbit Polyclonal Angiopoietin 2/ANG2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Human, Mouse samples. Cited in 42 publications.
View Alternative Names
Agpt2, Angpt2, Angiopoietin-2, ANG-2
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (AB8452)
ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- WB
Supplier Data
Western blot - Anti-Angiopoietin 2/ANG2 antibody (AB8452)
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt
All lanes:
Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab8452)
Predicted band size: 56 kDa
false
This image is courtesy of Marion Scharpfenecker 2002
Reactivity data
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.
Pathways
Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.
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Target data
Publications (42)
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Nature cardiovascular research 3:951-969 PubMed39155965
2024
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Experimental biology and medicine (Maywood, N.J.) 248:732-745 PubMed37354086
2023
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Neurology. Genetics 9:e200074 PubMed37152444
2023
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Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 54:791-801 PubMed36877445
2023
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Experimental & molecular medicine 55:470-484 PubMed36828931
2023
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Frontiers in oncology 13:1072802 PubMed36874142
2023
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Oxidative medicine and cellular longevity 2022:1792412 PubMed35340210
2022
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Frontiers in molecular biosciences 8:685466 PubMed34422901
2021
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Science translational medicine 13: PubMed34321321
2021
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International endodontic journal 54:1687-1698 PubMed33999433
2021
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