JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB8452

Anti-Angiopoietin 2/ANG2 antibody

3

(4 Reviews)

|

(42 Publications)

Rabbit Polyclonal Angiopoietin 2/ANG2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Mouse, Human, Mouse samples. Cited in 42 publications.

View Alternative Names

Agpt2, Angpt2, Angiopoietin-2, ANG-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (AB8452)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (AB8452)

ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-Angiopoietin 2/ANG2 antibody (AB8452)
  • WB

Supplier Data

Western blot - Anti-Angiopoietin 2/ANG2 antibody (AB8452)

Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.

Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab8452)

Predicted band size: 56 kDa

false

This image is courtesy of Marion Scharpfenecker 2002

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This reagent cross-reacts with human angiopoietin-2&#46; Partially cross-reactivity is noted with human angiopoietin-1&#46;</p>

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p>There is reaction with serum in the cell supernatants, which results in strong background and makes it difficult to see the angiopoietins in cell supernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Purification notes
Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: 98.5% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (By similarity). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression (By similarity). In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (By similarity). Involved in the regulation of lymphangiogenesis (By similarity).
See full target information Angpt2

Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:951-969 PubMed39155965

2024

Endothelial LATS2 is a suppressor of bone marrow fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kishor K Sivaraj,Paul-Georg Majev,Backialakshmi Dharmalingam,Silke Schröder,Bella Banjanin,Martin Stehling,Dagmar Zeuschner,Alfred Nordheim,Rebekka K Schneider,Ralf H Adams

Experimental biology and medicine (Maywood, N.J.) 248:732-745 PubMed37354086

2023

Epicatechin gallate promotes vascularization in co-culture of human osteoblasts and outgrowth endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Zhang,Miaoran Wang,Huiqing Qiu,Yusen Wei,Lu Zhou,Nannan Nian,Zhongli Shi,Dailun Hu,Bin Ma

Neurology. Genetics 9:e200074 PubMed37152444

2023

CircPDS5B Reduction Improves Angiogenesis Following Ischemic Stroke by Regulating MicroRNA-223-3p/NOTCH2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Kui,Zongyu Li,Guoyun Wang,Xuzhen Li,Feng Zhao,Yinming Jiao

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 54:791-801 PubMed36877445

2023

Helicobacter pylori infection induces abnormal expression of pro-angiogenic gene ANGPT2 and miR-203a in AGS gastric cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy Malespín-Bendaña,Rui M Ferreira,Marta T Pinto,Ceu Figueiredo,Warner Alpízar-Alpízar,Clas Une,Lucía Figueroa-Protti,Vanessa Ramírez

Experimental & molecular medicine 55:470-484 PubMed36828931

2023

An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone.

Applications

Unspecified application

Species

Unspecified reactive species

Eunhyeong Lee,Eun-Ah Lee,Eunji Kong,Haemin Chon,Melissa Llaiqui-Condori,Cheon Ho Park,Beom Yong Park,Nu Ri Kang,Jin-San Yoo,Hyun-Soo Lee,Hyung-Seok Kim,Sung-Hong Park,Seung-Won Choi,Dietmar Vestweber,Jeong Ho Lee,Pilhan Kim,Weon Sup Lee,Injune Kim

Frontiers in oncology 13:1072802 PubMed36874142

2023

infection induces gastric precancerous lesions and persistent expression of Angpt2, Vegf-A and in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy Malespín-Bendaña,Warner Alpízar-Alpízar,Lucía Figueroa-Protti,Ledis Reyes,Silvia Molina-Castro,Clas Une,Vanessa Ramírez-Mayorga

Oxidative medicine and cellular longevity 2022:1792412 PubMed35340210

2022

Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Yu,Qikun Liu,Yinguang Wang,Yuan Bao,Yongqiao Jiang,Mengwei Li,Zhiwei Li,Baodong Wang,Lei Yu,Shanxi Wang,Ming Shao,Hao Kang

Frontiers in molecular biosciences 8:685466 PubMed34422901

2021

Circular Ribonucleic Acid circFTO Promotes Angiogenesis and Impairs Blood-Retinal Barrier Targeting the miR-128-3p/Thioredoxin Interacting Protein Axis in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjin Guo,Feng Xiao,Wei Ren,Yikun Zhu,Qiujing Du,Qian Li,Xing Li

Science translational medicine 13: PubMed34321321

2021

Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Sun,Wei Chen,Robert J Torphy,Sheng Yao,Gefeng Zhu,Ronggui Lin,Roberta Lugano,Emily N Miller,Yuki Fujiwara,Li Bian,Linghua Zheng,Sudarshan Anand,Fan Gao,Weizhou Zhang,Sarah E Ferrara,Andrew E Goodspeed,Anna Dimberg,Xiao-Jing Wang,Barish H Edil,Carlton C Barnett,Richard D Schulick,Lieping Chen,Yuwen Zhu

International endodontic journal 54:1687-1698 PubMed33999433

2021

Impairment of the angiogenic process may contribute to lower success rate of root canal treatments in diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

José P Martinho,Ana Coelho,Bárbara Oliveiros,Salomé Pires,Ana Margarida Abrantes,Siri Paulo,Ana Catarina Carvalho,Eunice Carrilho,Anabela Paula,Lina Carvalho,Raquel Seiça,Maria Filomena Botelho,Carlos Miguel Marto,Gianrico Spagnuolo,Paulo Matafome,Manuel Marques Ferreira
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com