Skip to main content

Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823), expandable thumbnail
  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823), expandable thumbnail
  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823), expandable thumbnail
  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (AB259823), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Involved in the regulation of lymphangiogenesis (PubMed:32908006).

Alternative names

Angiopoietin-2, ANG-2, ANGPT2

Recommended products

Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24161-73
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Associated diseases and disorders

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823), expandable thumbnail

    Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
    Positive tissue: placenta, ovary (PMID:9204896)
    Negative control:skeletal muscle (PMID:9204896).
    Exposure time: 114 seconds

    All lanes: Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

    Lane 1: Rat ovary tissue lysate at 20 µg

    Lane 2: Rat placenta tissue lysate at 20 µg

    Lane 3: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 60-75 kDa

  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823), expandable thumbnail

    Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
    Positive tissue: placenta, ovary (PMID:9204896)
    Exposure time: 3 minutes

    All lanes: Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

    Lane 1: Mouse placenta tissue lysate at 20 µg

    Lane 2: Mouse ovary tissue lysate at 20 µg

    Lane 3: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 60-75 kDa

  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823), expandable thumbnail

    Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)

    Positive cell line: HUVEC (PMID:25857554)

    Negative control: HEK-293 (PMID:32908006)

    Exposure time: Lanes 1-2: 3 minutes; Lanes 3-4: 81 seconds.

    All lanes: Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 4: TF-1 treated with PNGase F whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 60-75 kDa

  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823), expandable thumbnail

    Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)

    Positive tissue: placenta (PMID:9204896)

    Exposure time: 48 seconds

    All lanes: Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution

    All lanes: Human placenta tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 56 kDa

    Observed band size: 60-75 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com