AB285368

Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Angiopoietin 2/ANG2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

Angiopoietin-2, ANG-2, ANGPT2

4 Images

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

This data was developed using ab259823, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive tissue : placenta (PMID : 9204896)

Exposure time : 48 seconds

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (<a href='/en-us/products/primary-antibodies/angiopoietin-2-ang2-antibody-epr24161-73-ab259823'>ab259823</a>) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

This data was developed using ab259823, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive cell line : HUVEC (PMID : 25857554)

Negative control : HEK-293 (PMID : 32908006)

Lanes 1-2 : 3 minutes; Lanes 3-4 : 81 seconds.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (<a href='/en-us/products/primary-antibodies/angiopoietin-2-ang2-antibody-epr24161-73-ab259823'>ab259823</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 4:

TF-1 treated with PNGase F whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

This data was developed using ab259823, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive tissue : placenta, ovary (PMID : 9204896)

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (<a href='/en-us/products/primary-antibodies/angiopoietin-2-ang2-antibody-epr24161-73-ab259823'>ab259823</a>) at 1/1000 dilution

Lane 1:

Mouse placenta tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Lab

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

This data was developed using ab259823, the same antibody clone in a different buffer formulation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29118335, PMID : 32908006)

Positive tissue : placenta, ovary (PMID : 9204896)

Negative control : skeletal muscle (PMID : 9204896).

Exposure time : 114 seconds

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (<a href='/en-us/products/primary-antibodies/angiopoietin-2-ang2-antibody-epr24161-73-ab259823'>ab259823</a>) at 1/1000 dilution

Lane 1:

Rat ovary tissue lysate at 20 µg

Lane 2:

Rat placenta tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 60-75 kDa

false

Unconjugated

Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24161-73

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab285368 is the carrier-free version of ab259823.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).

See full target information ANGPT2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (AB285368)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise