AB56301

Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]

Name: Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) | Abcam
Brand: Abcam Limited
SKU: AB56301
Price: 535 USD
Availability: InStock

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(9 Publications)

Mouse Monoclonal Angiopoietin 2/ANG2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANGPT2.

View Alternative Names

Angiopoietin-2, ANG-2, ANGPT2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (AB56301)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (AB56301)

ab56301 at 1/200 dilution, staining Angiopoietin 2/ANG2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Unknown

Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (AB56301)

The band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2/ANG2 due to the presence of an 18 amino acid signal peptide.

All lanes:

Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) at 1 µg/mL

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 56 kDa

Observed band size: 47 kDa,52 kDa,65 kDa

true

Exposure time: 4min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0020-1F29

Isotype

IgG2

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANGPT2.

O15123

Specificity

This antibody recognizes Angiopoietin 2/ANG2 in western blot without cross reactivity with Angiopoietin 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.

Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed : 15284220, PubMed : 19116766, PubMed : 19223473, PubMed : 9204896). Involved in the regulation of lymphangiogenesis (PubMed : 32908006).

See full target information ANGPT2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 28: PubMed37049657

2023

Utilizing Contrast-Enhanced Ultrasonography with Phosphatidylserine Microbubbles to Detect Placental Inflammation in Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel C Wilson,Jamie O Lo,Gabriel Romero Jimenez,Jonathan R Lindner,Ov D Slayden,Victoria H J Roberts

iScience 25:104407 PubMed35663028

2022

Neuregulin-1/ErbB4 signaling modulates HRP2-induced damage to brain cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Harbuzariu,Annette Nti,Keri Oxendine Harp,Juan C Cespedes,Adel Driss,Jonathan K Stiles

Cell reports 37:109996 PubMed34818544

2021

Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Ju Kim,Yi Zhao,Jae Kyung Myung,Joo Mi Yi,Min-Jung Kim,Su-Jae Lee

Molecular medicine reports 24: PubMed34036386

2021

miR‑135a‑5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Diao,Xing Xu,Bin Zhao,Guanghua Yang

Oncology letters 20:271 PubMed32989405

2020

Analysis of vascular-associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Gaofei Yin,Hongfei Liu,Hanyuan Duan,Zhigang Huang

Stem cell research & therapy 10:287 PubMed31547870

2019

miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Fei Zhou,Min-Juan Liu,Wei Wang,Sha Wu,Yu-Xin Huang,Guo-Bin Chen,Li-Min Liu,Dong-Xian Peng,Xue-Feng Wang,Xu-Zi Cai,Xiao-Xuan Li,Wan-Qin Feng,Ying Ma

International journal of cancer 145:2157-2169 PubMed30924128

2019

Fibulin-7 is overexpressed in glioblastomas and modulates glioblastoma neovascularization through interaction with angiopoietin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Susana de Vega,Akihide Kondo,Mario Suzuki,Hajime Arai,Shabierjiang Jiapaer,Hemragul Sabit,Mitsutoshi Nakada,Tomoko Ikeuchi,Muneaki Ishijima,Eri Arikawa-Hirasawa,Yoshihiko Yamada,Yasunori Okada

Scientific reports 8:15755 PubMed30361676

2018

Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yih-Jing Lee,Chia-Ying Ke,Ni Tien,Po-Kang Lin

Investigative ophthalmology & visual science 58:4332–4343 PubMed28806447

2017

Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yih-Jing Lee,Chien-Chao Chiu,Chia-Ying Ke,Ni Tien,Po-Kang Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (AB56301)

Western blot - Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (AB56301)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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