Goat Polyclonal Angiopoietin 4 antibody. Suitable for sELISA, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ANGPT4 aa 50-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANGPT4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y264
Reactivity data
Select an application| sELISA | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Capture at 1 μg/ml with ab93657 as Detection |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
Alternative names
ANG3, ANG4, ANGPT4, Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3
Recommended products
Goat Polyclonal Angiopoietin 4 antibody. Suitable for sELISA, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ANGPT4 aa 50-100.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANGPT4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y264
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiopoietin 4 also known as ANGPT4 or AGP4 is a secreted glycoprotein involved in vascular development. This protein has a molecular mass of around 56 kDa. ANGPT4 is expressed in a variety of tissues including the lung heart and kidney. It functions by interacting with the Tie receptors influencing vascular remodeling and angiogenesis. Angiopoietin 4 shares homology with other angiopoietins but has distinct regulatory roles compared to its counterparts.
- Biological function summary
Angiopoietin 4 regulates angiogenesis and vascular permeability. It acts independently and does not part of a known protein complex. ANGPT4 contributes to the restructuring of blood vessels by promoting endothelial cell survival and migration. By balancing the actions of other angiopoietins such as Angiopoietin 1 it supports the maturation and stabilization of vascular networks impacting overall vascular function and growth.
- Pathways
Angiopoietin 4 affects early morphogenetic signaling in vascular development. ANGPT4 operates within the angiopoietin-Tie signaling pathway important for angiogenesis and lymphangiogenesis. It interacts with Tie2 a receptor tyrosine kinase to mediate these effects. Within the pathway ANGPT4 coordinates with Angiopoietin 1 and Angiopoietin 2 modulating the balance between vessel stability and growth as required.
- Associated diseases and disorders
Angiopoietin 4 shows relevance to conditions like cancer and diabetic retinopathy. Abnormal expression or signaling of ANGPT4 can lead to defective vascular structures contributing to the pathology of these diseases. In cancer altered ANGPT4 and its interaction with Tie2 may disrupt normal angiogenesis facilitating tumor growth. Additionally in diabetic retinopathy ANGPT4 expression changes can exacerbate abnormal vessel formation highlighting its importance alongside Angiopoietin 2 in disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Sandwich ELISA - Anti-Angiopoietin 4 antibody (ab87877)
Standard Curve for Angiopoietin 4 dilution range 1pg/ml to 1ug/ml using Capture Antibody Goat polyclonal to Angiopoietin 4 (ab87877) at 1ug/ml and Detector Antibody Rabbit polyclonal to Angiopoietin 4 (ab93657) at 0.5ug/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 4 antibody (ab87877)
Immunohistochemistry on paraffin sections of human lung using ab87877 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 4 antibody (ab87877)
Immunohistochemistry on paraffin sections of human liver using ab87877 at 1/250 dilution.
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