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AB313126

Anti-Angiopoietin 4 antibody [EPR24087-66]

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Rabbit Recombinant Monoclonal Angiopoietin 4 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

ANG3, ANG4, ANGPT4, Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3

1 Images
Western blot - Anti-Angiopoietin 4 antibody [EPR24087-66] (AB313126)
  • WB

Supplier Data

Western blot - Anti-Angiopoietin 4 antibody [EPR24087-66] (AB313126)

ab313126 was shown to specifically react with Angiopoietin-4. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab313126 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Angiopoietin 4 antibody [EPR24087-66] (ab313126) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 2:

Human kidney tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Secondary

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

Observed band size: 56 kDa,37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24087-66

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin 4 also known as ANGPT4 or AGP4 is a secreted glycoprotein involved in vascular development. This protein has a molecular mass of around 56 kDa. ANGPT4 is expressed in a variety of tissues including the lung heart and kidney. It functions by interacting with the Tie receptors influencing vascular remodeling and angiogenesis. Angiopoietin 4 shares homology with other angiopoietins but has distinct regulatory roles compared to its counterparts.
Biological function summary

Angiopoietin 4 regulates angiogenesis and vascular permeability. It acts independently and does not part of a known protein complex. ANGPT4 contributes to the restructuring of blood vessels by promoting endothelial cell survival and migration. By balancing the actions of other angiopoietins such as Angiopoietin 1 it supports the maturation and stabilization of vascular networks impacting overall vascular function and growth.

Pathways

Angiopoietin 4 affects early morphogenetic signaling in vascular development. ANGPT4 operates within the angiopoietin-Tie signaling pathway important for angiogenesis and lymphangiogenesis. It interacts with Tie2 a receptor tyrosine kinase to mediate these effects. Within the pathway ANGPT4 coordinates with Angiopoietin 1 and Angiopoietin 2 modulating the balance between vessel stability and growth as required.

Angiopoietin 4 shows relevance to conditions like cancer and diabetic retinopathy. Abnormal expression or signaling of ANGPT4 can lead to defective vascular structures contributing to the pathology of these diseases. In cancer altered ANGPT4 and its interaction with Tie2 may disrupt normal angiogenesis facilitating tumor growth. Additionally in diabetic retinopathy ANGPT4 expression changes can exacerbate abnormal vessel formation highlighting its importance alongside Angiopoietin 2 in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
See full target information ANGPT4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com