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AB115798

Anti-Angiopoietin-like 4/ANGPTL4 antibody

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(7 Publications)

Rabbit Polyclonal Angiopoietin-like 4/ANGPTL4 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ANGPTL4.

View Alternative Names

ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein

2 Images
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (AB115798)
  • WB

Supplier Data

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (AB115798)

All lanes:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab115798)

Lane 1:

Human Placenta Tissue Lysate at 50 µg

Lane 2:

SMMC Whole Cell Lysate at 40 µg

Lane 3:

HeLa Whole Cell Lysate at 40 µg

Predicted band size: 45 kDa

false

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (AB115798)
  • WB

Unknown

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (AB115798)

All lanes:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab115798)

Lane 1:

Smmc Whole Cell Lysate

Lane 2:

A549 Whole Cell Lysate

Lane 3:

U87 Whole Cell Lysate

Lane 4:

293T Whole Cell Lysate

Lane 5:

SGC Whole Cell Lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ANGPTL4. The exact immunogen used to generate this antibody is proprietary information.

Q9BY76

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>The detection limit for Angiopoietin-like 4/ANGPTL4 is approximately 2.5ng/lane under non-reducing and reducing conditions.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.
Biological function summary

ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.

Pathways

The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.

ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed : 14583458, PubMed : 17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed : 17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity).. ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed : 19270337, PubMed : 21398697).
See full target information ANGPTL4
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:8348-8360 PubMed38159259

2023

ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Qihao Hu,Shi Chen,Yukun Li,Teng Hu,Jianpeng Hu,Cheng Wang,Fei Yang,Xiang Yang,Feng Zhou,Zhengdong Liu,Wei Xu,Ji Zhang

The Journal of experimental medicine 220: PubMed36269299

2022

Primary tumor-derived systemic nANGPTL4 inhibits metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Corinne Hübers,Ashik Ahmed Abdul Pari,Denise Grieshober,Martin Petkov,Alexander Schmidt,Tatjana Messmer,Christian Moritz Heyer,Sebastian Schölch,Stephanie S Kapel,Nicolas Gengenbacher,Mahak Singhal,Benjamin Schieb,Claudine Fricke,Rainer Will,Kim Remans,Jochen Sven Utikal,Christoph Reissfelder,Matthias Schlesner,Kairbaan M Hodivala-Dilke,Sander Kersten,Sergij Goerdt,Hellmut G Augustin,Moritz Felcht

BMC cancer 20:878 PubMed32928141

2020

ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Cai,Hang Yang,Ke-Feng Wang,Tan-Huan Chen,Wen-Qi Jiang,Yan-Xia Shi

Frontiers in immunology 10:950 PubMed31130950

2019

Reduced Angiopoietin-Like 4 Expression in Multiple Sclerosis Lesions Facilitates Lipid Uptake by Phagocytes via Modulation of Lipoprotein-Lipase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Alwin Kamermans,Merel Rijnsburger,Ananya Chakraborty,Susanne van der Pol,Helga E de Vries,Jack van Horssen

Scientific reports 8:11954 PubMed30097618

2018

Enzyme-modified non-oxidized LDL (ELDL) induces human coronary artery smooth muscle cell transformation to a migratory and osteoblast-like phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Bijoy Chellan,Elizabeth Rojas,Chunling Zhang,Marion A Hofmann Bowman

Oncotarget 8:54966-54977 PubMed28903395

2017

TGF-β2-induced ANGPTL4 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Ming Qian,Hao Cao,Qi Jia,Zhipeng Wu,Xinghai Yang,Tianyi Ma,Haifeng Wei,Tianrui Chen,Jianru Xiao

PloS one 8:e73146 PubMed24039874

2013

c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype.

Applications

WB

Species

Unspecified reactive species

Victoria Florea,Nithya Bhagavatula,Gordana Simovic,Francisco Y Macedo,Ricardo A Fock,Claudia O Rodrigues
View all publications
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