Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) is a rabbit polyclonal antibody that is used to detect Angiopoietin-like 4/ANGPTL4 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 20 publications
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- Aging 16:8348-83602023ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQihao Hu,Shi Chen,Yukun Li,Teng Hu,Jianpeng Hu,Cheng Wang,Fei Yang,Xiang Yang,Feng Zhou,Zhengdong Liu,Wei Xu,Ji ZhangPubMed 38159259
- Iranian journal of basic medical sciences 26:587-5932023ANGPTL4 suppresses the profibrogenic functions of atrial fibroblasts induced by angiotensin II by up-regulating PPARγ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Zhu,Xiaogang Zhang,Wei Gu,Hanjun Zhao,Shuwen Hao,Zhongping NingPubMed 37051105
- The journal of gene medicine 25:e35062023STAT2-induced linc02231 promotes tumorigenesis and angiogenesis through modulation of hnRNPA1/ANGPTL4 in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufen Xu,Ying Fang,Lisha Chang,Yibo Bian,Yuting Wang,Jie Ding,Yang Wang,Yangyang Zhang,Juan Pu,Keming WangPubMed 36994700
- Pathophysiology : the official journal of the International Society for Pathophysiology 29:9-232022Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZaleha Kamaludin,Alaa Siddig,Najib Majdi Yaacob,Alfred K Lam,Wan Faiziah Wan Abdul RahmanPubMed 35366286
- The British journal of nutrition 129:1657-16662021Oleic and palmitic acids induce hepatic angiopoietin-like 4 expression predominantly via PPAR- in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojun Xiang,Shangzhe Han,Dan Xu,Qiuchi Chen,Renlei Ji,Zengqi Zhao,Jianlong Du,Kangsen Mai,Qinghui AiPubMed 34556193
- Cardiology research and practice 2021:99353102021ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Zhu,Xiaogang Zhang,Xinpeng Cong,Luoning Zhu,Zhongping NingPubMed 34422410
- Journal of Cancer 12:5473-54852021DNA methylation mediated down-regulation of ANGPTL4 promotes colorectal cancer metastasis by activating the ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKunning Zhang,Zhiwei Zhai,Sanshui Yu,Yu TaoPubMed 34405010
- International endodontic journal 54:1687-16982021Impairment of the angiogenic process may contribute to lower success rate of root canal treatments in diabetes mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé P Martinho,Ana Coelho,Bárbara Oliveiros,Salomé Pires,Ana Margarida Abrantes,Siri Paulo,Ana Catarina Carvalho,Eunice Carrilho,Anabela Paula,Lina Carvalho,Raquel Seiça,Maria Filomena Botelho,Carlos Miguel Marto,Gianrico Spagnuolo,Paulo Matafome,Manuel Marques FerreiraPubMed 33999433
- Cancer cell international 21:1712021Deregulation of angiopoietin-like 4 slows ovarian cancer progression through vascular endothelial growth factor receptor 2 phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxian Wu,Jinghai Gao,Xiaojun LiuPubMed 33726754
- Cancer management and research 12:10521-105302020LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyi Chen et. al.PubMed 33122950
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ANGPTL4. Database link Q9BY76
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/200 | Notes - |
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed:17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity). ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed:19270337, PubMed:21398697).
Alternative names
ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein
Recommended products
Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) is a rabbit polyclonal antibody that is used to detect Angiopoietin-like 4/ANGPTL4 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ANGPTL4. Database link Q9BY76
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Angiopoietin-like 4/ANGPTL4?
Anti-Angiopoietin-like 4/ANGPTL4 (ab196746) specifically detects a band for Angiopoietin-like 4/ANGPTL4 (UniProt: Q9BY76) at a molecular weight of 45kDa.
Trusted by the scientific community
Anti-Angiopoietin-like 4/ANGPTL4 (ab196746) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.
- Biological function summary
ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.
- Pathways
The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.
- Associated diseases and disorders
ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746)
Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746)
All lanes: Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) at 1/500 dilution
All lanes: Mouse skeletal muscle extract
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746)
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746)
Immunofluorescent analysis of A549 cells labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/50 dilution. Blue: DAPI for nuclear staining.
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