AB206420

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Angiopoietin-like 4/ANGPTL4 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution

All lanes:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 35 kDa,50 kDa

false

Exposure time: 1min

Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 3 minutes; Lane 5 : 5 seconds.

Angiopoietin-like 4/ANGPTL4 can be cleaved at 161-164 site and this immunogen recognize full length 50KD and C-Terminal fragment 35KD. (PMID : 19270337; PMID : 10862772).

Lanes 1 - 3:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/2000 dilution

Lanes 4 - 5:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

THP-1 (human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 4:

Human kidney lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 4 - 5:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 35 kDa,50 kDa

false

Carrier free

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19873

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/700", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.

Biological function summary

ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.

Pathways

The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.

ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed : 14583458, PubMed : 17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed : 17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity).. ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed : 19270337, PubMed : 21398697, PubMed : 27929370, PubMed : 29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed : 19270337, PubMed : 21398697).

See full target information ANGPTL4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 9:1003914 PubMed36275794

2022

Establishment of a ccRCC patient-derived chick chorioallantoic membrane model for drug testing.

Applications

Unspecified application

Species

Unspecified reactive species

Martine Charbonneau,Kelly Harper,Karine Brochu-Gaudreau,Alexis Perreault,Patrick P McDonald,Nadia Ekindi-Ndongo,Claudio Jeldres,Claire M Dubois

iScience 24:103170 PubMed34646996

2021

Excipient of paclitaxel induces metabolic dysregulation and unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Dai,Xiaolin Liu,Tao He,Chao Yang,Jinfeng Jiang,Yin Fang,Zhoukai Fu,Yuan Yuan,Shujun Bai,Tong Qiu,Rutie Yin,Ping Ding,Jie Chen,Qintong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (AB206420)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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