Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4//ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206420).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206420).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206420).

• WB

Lab

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)

This data was developed using ab206420, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 2:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (<a href='/en-us/products/primary-antibodies/angiopoietin-like-4-angptl4-antibody-epr19873-ab206420'>ab206420</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-epr20547-chip-grade-ab213204'>ab213204</a>) at 1/5000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ANGPTL4 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,50 kDa

false

Exposure time: 3s