Rabbit Recombinant Monoclonal Angiopoietin-like 4/ANGPTL4 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--immunocytochemistry-immunofluorescence-img70576.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)")
![Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--flow-cytometry-intracellular-img80283.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)")
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--immunocytochemistry-immunofluorescence-img42365.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (AB240373)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed:17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity). ANGPTL4 N-terminal chain. Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed:19270337, PubMed:21398697).
Alternative names
ARP4, HFARP, PGAR, PP1158, PSEC0166, UNQ171/PRO197, ANGPTL4, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein
Recommended products
Rabbit Recombinant Monoclonal Angiopoietin-like 4/ANGPTL4 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19873
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240373 is the carrier-free version of Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiopoietin-like 4 also known as ANGPTL4 like4 or ANGPTL4 protein is a glycoprotein with an approximate mass of 50 kDa. It plays a role in lipid metabolism by inhibiting lipoprotein lipase which reduces the breakdown of triglycerides. ANGPTL4 protein is secreted by liver adipose tissue and placenta showing expression in response to fasting and during low insulin states. This expression pattern connects its function to energy homeostasis and metabolic regulation.
- Biological function summary
ANGPTL4 protein influences processes such as lipid metabolism angiogenesis and wound healing. It does not integrate into a large protein complex but functions independently to exert its effects. Inhibition of lipoprotein lipase by ANGPTL4 decreases adipose tissue fat storage and modulates plasma lipid levels. This modulation plays a critical role in maintaining energy balance and glucose homeostasis particularly under metabolic stress conditions.
- Pathways
The functionality of ANGPTL4 protein intersects with the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and the glucose homeostasis pathway. It interacts with other proteins such as PPAR-alpha and PPAR-gamma which regulate genes involved in fatty acid oxidation and lipid transport. This interaction influences the body's response to fasting and caloric restriction by altering lipid metabolism and ensuring efficient energy use.
- Associated diseases and disorders
ANGPTL4 protein correlates with cardiovascular diseases and cancer. Elevated levels of ANGPTL4 associate with an increased risk for atherosclerosis due to its role in lipid metabolism disturbance. In cancer particularly gastric cancer ANGPTL4 influences tumor metastasis by promoting angiogenesis. Its effects on these diseases link to proteins like VEGF in angiogenesis and other metabolic regulators in cardiovascular conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--immunocytochemistry-immunofluorescence-img70576.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)")
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).
Alpha-Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420).
![Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--flow-cytometry-intracellular-img80283.jpg "Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)")
Flow Cytometry (Intracellular) - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4//ANGPTL4 with Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420).
![Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373), expandable thumbnail](https://content.abcam.com/products/images/angiopoietin-like-4-angptl4-antibody-epr19873-bsa-and-azide-free-ab240373--immunocytochemistry-immunofluorescence-img42365.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)")
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] - BSA and Azide free (ab240373)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).
Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] ab206420).
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