Anti-Angiopoietin-like 4/ANGPTL4 antibody [RP23040038]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [RP23040038] (AB308049)

For immunofluorescence analysis, 70% confluent log phase HUVEC cells treated with 150uM of Cobalt Chloride for 24 h were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature for detection of Angiopoietin-like 4/ANGPTL4 using ab308049 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution Panel d represents the merged image showing cytoplasmic localization Panel e is untreated cell with no signal Panel f represents control cells with no primary antibody to assess background The images were captured at 60X magnification

• WB

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Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [RP23040038] (AB308049)

Western blot analysis of Angiopoietin-like 4/ANGPTL4 in whole cell extracts of serum-starved 3T3 L1 cells treated with Insulin (100 ng/mL, 15 min) using ab308049 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~45 kDa.

All lanes:

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [RP23040038] (ab308049) at 2 µg/mL

All lanes:

Insulin treated, serum-starved 3T3 L1 cells

Observed band size: 45 kDa

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