Anti-Angiostatin antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (AB2904)

Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2904 in 3% BSA-PBS at a dilution of 1 : 20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• WB

Unknown

Western blot - Anti-Angiostatin antibody (AB2904)

All lanes:

Western blot - Anti-Angiostatin antibody (ab2904) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

Human Plasma Total Protein Lysate at 10 µg

Lane 3:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 27 kDa,38 kDa,48 kDa,50 kDa

true

Exposure time: 12min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (AB2904)

Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab2904 in 3% BSA-PBS at a dilution of 1 : 20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.