Rabbit Recombinant Monoclonal Angiotensin Converting Enzyme 1 antibody. Suitable for IHC-P, ELISA, WB and reacts with Human samples. Cited in 14 publications.
Images
![ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--elisa-img137899.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17732.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118606.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762)")
![Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--western-blot-img417457.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (AB75762)")
Publications
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- Iranian journal of pharmaceutical research : IJPR 20:316-3322021Inhibition of HMGB1 Might Enhance the Protective Effect of Taxifolin in Cardiomyocytes via PI3K/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErjun Feng,Jian Wang,Xinwei Wang,Zhenguo Wang,Xiaochu Chen,Xu Zhu,Wenli HouPubMed 34567165
- Cell death & disease 11:6792020Aldosterone from endometrial glands is benefit for human decidualization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Yun Li,Zhuo Song,Ya-Ping Yan,Bo Li,Min-Jie Song,Yue-Fang Liu,Zhen-Shan Yang,Meng-Yuan Li,Ai-Xia Liu,Song Quan,Zeng-Ming YangPubMed 32826848
- Experimental cell research 393:1120862020iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiyu Liu et. al.PubMed 32416091
- Journal of the American Heart Association 9:e0147612020Localized Antileptin Therapy Prevents Aortic Root Dilatation and Preserves Left Ventricular Systolic Function in a Murine Model of Marfan Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSudeshna Fisch et. al.PubMed 32378446
- Aging cell 18:e130042019Wnt/β-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Miao et. al.PubMed 31318148
- International journal of molecular sciences 20:2019Cardioprotection Conferred by Sitagliptin Is Associated with Reduced Cardiac Angiotensin II/Angiotensin-(1-7) Balance in Experimental Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliana Isa Beraldo et. al.PubMed 31010001
- Biochimica et biophysica acta. Molecular basis of 1865:1313-13222019Wnt/β-catenin regulates blood pressure and kidney injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangxiang Xiao et. al.PubMed 30710617
- The Journal of experimental medicine 215:2554-25662018Lupus antibodies induce behavioral changes mediated by microglia and blocked by ACE inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacquelyn Nestor et. al.PubMed 30185634
- Oncotarget 8:24548-245632017Angiotensin-converting enzyme 2 overexpression protects against doxorubicin-induced cardiomyopathy by multiple mechanisms in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Ma,Jing Kong,Yu-Lin Wang,Jun-Long Li,Nai-Hao Hei,Xin-Ran Cao,Jing-Jing Yang,Wen-Jiang Yan,Wen-Jing Liang,Hong-Yan Dai,Bo DongPubMed 28445944
- Cardiovascular research 113:760-7692017ELABELA-APJ axis protects from pressure overload heart failure and angiotensin II-induced cardiac damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeruki Sato,Chitose Sato,Ayumi Kadowaki,Hiroyuki Watanabe,Lena Ho,Junji Ishida,Tomokazu Yamaguchi,Akinori Kimura,Akiyoshi Fukamizu,Josef M Penninger,Bruno Reversade,Hiroshi Ito,Yumiko Imai,Keiji KubaPubMed 28371822
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | IP | ELISA | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.002-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Alternative names
Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, DCP1, DCP, ACE
Recommended products
Rabbit Recombinant Monoclonal Angiotensin Converting Enzyme 1 antibody. Suitable for IHC-P, ELISA, WB and reacts with Human samples. Cited in 14 publications.
Alternative names
Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, DCP1, DCP, ACE
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR2757
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Angiotensin Converting Enzyme 1 also known as ACE1 or ACE is an important enzyme in the renin-angiotensin system. This enzyme exhibits a significant role in blood pressure regulation. ACE1 is a zinc-metallopeptidase with a molecular weight of approximately 130 kDa. It converts angiotensin I into the potent vasoconstrictor angiotensin II a critical function in cardiovascular physiology. ACE1 is widely expressed in endothelial cells particularly in the lungs kidneys and the small intestine.
- Biological function summary
The enzyme generates angiotensin II by cleaving angiotensin I. Angiotensin II an important effector peptide impacts cardiovascular and renal systems influencing vasoconstriction and fluid balance. While not directly forming a complex ACE1's activity increases the potency of angiotensin II which binds to angiotensin II receptors to exert its effects therefore indirectly forming a functional signaling complex.
- Pathways
ACE1 plays a central role in the renin-angiotensin system and the kallikrein-kinin system. The enzyme's activity boosts angiotensin II production which connects it to the regulation of blood pressure via the renin-angiotensin pathway. ACE1 also indirectly interacts with proteins like bradykinin by degrading them modulating kinin-related functions and influencing inflammation and tension in vascular smooth muscle.
- Associated diseases and disorders
Understanding ACE1 is important for addressing hypertension and congestive heart failure. ACE1's conversion of angiotensin I to angiotensin II means overactivity can cause elevated blood pressure leading to hypertension. This makes ACE inhibitors such as lisinopril and ramipril therapeutic for these conditions. Furthermore its connection with aldosterone production places ACE1 in relevance to heart failure as excessive aldosterone can cause detrimental remodeling of cardiac tissue.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--elisa-img137899.jpg "ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)")
ELISA - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ELISA analysis of Human ACE (angiotensin converting enzyme) (membrane form only) recombinant protein at 250ng/ml with ab75762. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17732.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--western-blot-img70802.gif "Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)")
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
All lanes: Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution
Lane 1: fetal kidney lysate at 10 µg
Lane 2: fetal heart lysate at 10 µg
Lane 3: fetal lung lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 150 kDa
Observed band size: 195 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118606.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762), expandable thumbnail](https://content.abcam.com/products/images/angiotensin-converting-enzyme-1-antibody-epr2757-ab75762--western-blot-img417457.jpg "Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)")
Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab75762 was shown to bind specifically to Angiotensin Converting Enzyme 1. A band was observed at 170 kDa in wild-type SKNFI cell lysates with no signal observed at this size in Ace knockout cell line. To generate this image, wild-type and Ace knockout SKNFI cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/500 dilution
Lane 1: SKNFI cell lysate at 20 µg
Lane 2: Ace knockout SKNFI cell lysate at 20 µg
Lane 3: HUVEC cell lysate at 20 µg
SecondaryAll lanes: Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 170 kDa
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