AB59018

Anti-Angiotensin II Type 1 Receptor antibody

Name: Anti-Angiotensin II Type 1 Receptor antibody (ab59018) | Abcam
Brand: Abcam Limited
SKU: AB59018

(1 Review)

(5 Publications)

Goat Polyclonal Angiotensin II Type 1 Receptor antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human AGTR1 aa 200-250.

View Alternative Names

AGTR1A, AGTR1B, AT2R1, AT2R1B, AGTR1, Type-1 angiotensin II receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1 receptor

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human AGTR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P30556

Specificity

All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).

Preliminary testing was unsuccessful on Rat Liver in Western blot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Angiotensin II Type 1 Receptor also known as AGTR1 AT1 receptor or Angiotensin Receptor 1 is a G protein-coupled receptor with a mass of approximately 45 kDa. This receptor binds to the angiotensin II peptide playing a central role in regulating blood pressure. It is broadly expressed in tissues such as vascular smooth muscle cells cardiac myocytes kidneys and brain. The AT1 receptor mediates vasoconstriction aldosterone secretion and cellular growth through its interaction with angiotensin II impacting cardiovascular functions.

Biological function summary

The Angiotensin II Type 1 Receptor contributes significantly to the regulation of cardiovascular homeostasis. It forms part of a complex signaling system involving G proteins phospholipase C and other downstream effectors. This receptor elicits responses important for maintaining vascular tone and fluid balance. By activating intracellular signaling cascade it plays an essential role in sodium reabsorption and systemic blood pressure maintenance. The receptor's expression and activity represent an important component in cardiovascular function.

Pathways

The AT1 receptor participates actively in the renin-angiotensin system (RAS) and is a significant player in the regulation of blood pressure. It interacts with angiotensin converting enzyme (ACE) and angiotensin II within this pathway. Additionally the receptor is linked to the mitogen-activated protein kinase (MAPK) pathway which further influences cell proliferation and vascular remodeling. These interactions highlight the receptor's involvement in vascular smooth muscle cell function and the broader implications for cardiovascular health.

The Angiotensin II Type 1 Receptor is closely associated with conditions such as hypertension and heart failure. Dysfunction or overactivity of this receptor contributes to elevated blood pressure and compromised cardiac function. The AT1 receptor's connection to ACE highlights its therapeutic relevance as many antihypertensive treatments target this interaction. By modulating the receptor's activity these treatments aim to alleviate symptoms and reduce the risk of cardiovascular events.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for angiotensin II, a vasoconstricting peptide, which acts as a key regulator of blood pressure and sodium retention by the kidney (PubMed : 15611106, PubMed : 1567413, PubMed : 25913193, PubMed : 26420482, PubMed : 30639100, PubMed : 32079768, PubMed : 8987975). The activated receptor in turn couples to G-alpha proteins G(q) (GNAQ, GNA11, GNA14 or GNA15) and thus activates phospholipase C and increases the cytosolic Ca(2+) concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C (PubMed : 15611106).. (Microbial infection) During SARS coronavirus-2/SARS-CoV-2 infection, it is able to recognize and internalize the complex formed by secreted ACE2 and SARS-CoV-2 spike protein through DNM2/dynamin 2-dependent endocytosis.

See full target information AGTR1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Circulation research 123:1080-1090 PubMed30355157

2018

Response Gene to Complement 32 Maintains Blood Pressure Homeostasis by Regulating α-Adrenergic Receptor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ming Tang,Ning Shi,Kun Dong,Scott A Brown,Amanda E Coleman,Matthew A Boegehold,Shi-You Chen

BMC physiology 15:2 PubMed25971747

2015

Antenatal maternal low protein diet: ACE-2 in the mouse lung and sexually dimorphic programming of hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi Goyal,Jonathan Van-Wickle,Dipali Goyal,Lawrence D Longo

Journal of the renin-angiotensin-aldosterone system : JRAAS 14:137-45 PubMed22898440

2012

Antenatal maternal protein deprivation: sexually dimorphic programming of the pancreatic renin-angiotensin system.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi Goyal,Christine Wong,Jonathan Van Wickle,Lawrence D Longo

Placenta 32:134-9 PubMed21130492

2010

Antenatal maternal hypoxic stress: adaptations of the placental renin-angiotensin system in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

R Goyal,R Lister,A Leitzke,D Goyal,C P Gheorghe,L D Longo

Reproductive sciences (Thousand Oaks, Calif.) 18:180-9 PubMed20978179

2010

Antenatal maternal hypoxic stress: adaptations in fetal lung Renin-Angiotensin system.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi Goyal,Arthur Leitzke,Dipali Goyal,Ciprian P Gheorghe,Lawrence D Longo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Angiotensin II Type 1 Receptor antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise