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Rabbit Recombinant Monoclonal Angiotensin II Type 1 Receptor antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Human, Mouse, Human, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAWBIHC-P
Human
Expected
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Rat
Expected
Tested
Not recommended
Recombinant full length protein - Human
Tested
Tested
Not recommended

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Recombinant full length protein - Human, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant full length protein - Human

Dilution info

-

Notes

-

Target data

Function

Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.(Microbial infection) During SARS coronavirus-2/SARS-CoV-2 infection, it is able to recognize and internalize the complex formed by secreted ACE2 and SARS-CoV-2 spike protein through DNM2/dynamin 2-dependent endocytosis.

Alternative names

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Rabbit Recombinant Monoclonal Angiotensin II Type 1 Receptor antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Human, Mouse, Human, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3873

Purification technique

Affinity purification Protein A

Specificity

The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab239995 is the carrier-free version of ab124734.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

The Angiotensin II Type 1 Receptor contributes significantly to the regulation of cardiovascular homeostasis. It forms part of a complex signaling system involving G proteins phospholipase C and other downstream effectors. This receptor elicits responses important for maintaining vascular tone and fluid balance. By activating intracellular signaling cascade it plays an essential role in sodium reabsorption and systemic blood pressure maintenance. The receptor's expression and activity represent an important component in cardiovascular function.

Activity summary

The Angiotensin II Type 1 Receptor also known as AGTR1 AT1 receptor or Angiotensin Receptor 1 is a G protein-coupled receptor with a mass of approximately 45 kDa. This receptor binds to the angiotensin II peptide playing a central role in regulating blood pressure. It is broadly expressed in tissues such as vascular smooth muscle cells cardiac myocytes kidneys and brain. The AT1 receptor mediates vasoconstriction aldosterone secretion and cellular growth through its interaction with angiotensin II impacting cardiovascular functions.

Pathways

The AT1 receptor participates actively in the renin-angiotensin system (RAS) and is a significant player in the regulation of blood pressure. It interacts with angiotensin converting enzyme (ACE) and angiotensin II within this pathway. Additionally the receptor is linked to the mitogen-activated protein kinase (MAPK) pathway which further influences cell proliferation and vascular remodeling. These interactions highlight the receptor's involvement in vascular smooth muscle cell function and the broader implications for cardiovascular health.

Associated diseases and disorders

The Angiotensin II Type 1 Receptor is closely associated with conditions such as hypertension and heart failure. Dysfunction or overactivity of this receptor contributes to elevated blood pressure and compromised cardiac function. The AT1 receptor's connection to ACE highlights its therapeutic relevance as many antihypertensive treatments target this interaction. By modulating the receptor's activity these treatments aim to alleviate symptoms and reduce the risk of cardiovascular events.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995), expandable thumbnail

    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734) at 1/2000 dilution

    Lane 1: C2C12 cell lysate at 20 µg

    Lane 2: PC-12 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 50 kDa, 57 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995), expandable thumbnail

    Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995), expandable thumbnail

    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734) at 1/2000 dilution

    All lanes: HT-1080 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/10000 dilution

    Predicted band size: 15 kDa, 41 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995), expandable thumbnail

    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734) at 1/1000 dilution

    Lane 1: C2C12 cell lysate at 10 µg

    Lane 2: PC12 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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