AB108334

Anti-Angiotensinogen antibody [EPR2931]

Name: Anti-Angiotensinogen antibody [EPR2931] (ab108334) | Abcam
Brand: Abcam Limited
SKU: AB108334
Price: 405 USD
Availability: InStock
Rating: 3 (4 reviews)

RabMAb
Recombinant
What is this?

(4 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal Angiotensinogen antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 12 publications.

View Alternative Names

SERPINA8, AGT, Angiotensinogen, Serpin A8

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

Lab

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

Blocking buffer for the first lanes 1-5 : 5% NFDM/TBST; the second lanes 1-5 : BSA/TBST.

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

Lane 1:

Mouse kidney whole tissue lysate at 10 µg

Lane 2:

Rat kidney whole tissue lysate at 10 µg

Lane 3:

Human kidney whole tissue lysate at 10 µg

Lane 4:

Mouse liver whole tissue lysate at 10 µg

Lane 5:

Rat liver whole tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/2000 dilution

Predicted band size: 53 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

Lane 1 blocking buffer : NFDM/TBST

Lane 2 blocking buffer : BSA/TBST

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

All lanes:

Human fetal liver whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) peroxidase conjugated at 1/2000 dilution

Predicted band size: 53 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

Lane 1:

Human fetal artery lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

293T lysate at 10 µg

Lane 6:

Human plasma lysate at 10 µg

Predicted band size: 53 kDa

false

Carrier free

Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2931

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Block with 5% BSA", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Block with 5% BSA", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Block with 5% BSA", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.

Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed : 10619573, PubMed : 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed : 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).

See full target information AGT

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 9:943986 PubMed35957853

2022

Optic nerve head astrocytes contribute to vascular associated effects.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmin Dong,Yue Fu,Xiaobing Qian,Leilei Lin,Yongguang Yuan,Yujie Li,Wanwen Shao,Qianying Gao

Oxidative medicine and cellular longevity 2021:3225439 PubMed34413926

2021

Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqiang Sun,Xiaofei Sun,Jingchuan Sun,Yan Jiang,Feng Lin,Fanqi Kong,Fudong Li,Jian Zhu,Le Huan,Bing Zheng,Yuan Wang,Weiguo Zou,Lu Gao,Ximing Xu,Jiangang Shi

Molecular medicine reports 23: PubMed33495829

2021

Bioinformatics analysis identifies biomarkers associated with poor prognosis in diffuse‑type gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Chao Yu,Yuanguang Cheng,Fangchao Du,Gang Wen

Journal of cellular biochemistry 121:25-42 PubMed31433522

2019

AT1R knockdown confers cardioprotection against sepsis-induced myocardial injury by inhibiting the MAPK signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Yu-Chao Yin,Xiang Ji,Bo Zhang,Sheng Wu,Xiao-Zhe Wu,Hong Li,Ya-Dan Li,Ya-Ling Ma,Yu Wang,Hai-Tao Li,Bin Zhang,Di Wu

American journal of physiology. Heart and circulatory physiology 312:H968-H979 PubMed28283551

2017

A novel role for miR-133a in centrally mediated activation of the renin-angiotensin system in congestive heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Neeru M Sharma,Shyam S Nandi,Hong Zheng,Paras K Mishra,Kaushik P Patel

Diabetes 64:519-28 PubMed25213336

2014

Tuberous sclerosis complex 1-mechanistic target of rapamycin complex 1 signaling determines brown-to-white adipocyte phenotypic switch.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Xiang,He Lan,Hong Tang,Fang Yuan,Yanhui Xu,Jing Zhao,Yin Li,Weizhen Zhang

Behavioural brain research 272:314-23 PubMed25046593

2014

Involvement of the brain renin-angiotensin system (RAS) in the neuroadaptive responses induced by amphetamine in a two-injection protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Constanza Paz,Natalia Andrea Marchese,Maria Mercedes Stroppa,Nelia Marta Gerez de Burgos,Hans Imboden,Gustavo Baiardi,Liliana Marina Cancela,Claudia Bregonzio

Journal of molecular and cellular cardiology 75:25-39 PubMed24976017

2014

Angiotensin II-regulated microRNA 483-3p directly targets multiple components of the renin-angiotensin system.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline R Kemp,Hamiyet Unal,Russell Desnoyer,Hong Yue,Anushree Bhatnagar,Sadashiva S Karnik

The Journal of endocrinology 221:215-27 PubMed24756097

2014

Downregulation of the ACE2/Ang-(1-7)/Mas axis in transgenic mice overexpressing GH.

Applications

Unspecified application

Species

Mouse

Marina C Muñoz,Valeria Burghi,Johanna G Miquet,Jorge F Giani,Ricardo D Banegas,Jorge E Toblli,Yimin Fang,Feiya Wang,Andrzej Bartke,Fernando P Dominici

PloS one 9:e88269 PubMed24505458

2014

Long-term effects of maternal diabetes on blood pressure and renal function in rat male offspring.

Applications

Species

Owl

Jie Yan,Xin Li,Rina Su,Kai Zhang,Huixia Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Angiotensinogen antibody [EPR2931]

Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

Western blot - Anti-Angiotensinogen antibody [EPR2931] (AB108334)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise