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Rabbit Recombinant Monoclonal Angiotensinogen antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869), expandable thumbnail
  • Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869), expandable thumbnail
  • Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Expected
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Block with 5% BSA.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

Block with 5% BSA.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed:10619573, PubMed:17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed:1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity). Angiotensin-3. Stimulates aldosterone release. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).

Alternative names

SERPINA8, AGT, Angiotensinogen, Serpin A8

Recommended products

Rabbit Recombinant Monoclonal Angiotensinogen antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2931
Purification technique
Affinity purification Protein A
Specificity

This antibody is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab215869 is the carrier-free version of Anti-Angiotensinogen antibody [EPR2931] ab108334.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.

Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Associated diseases and disorders

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869), expandable thumbnail

    Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869)

    This data was developed using Anti-Angiotensinogen antibody [EPR2931] ab108334, the same antibody clone in a different buffer formulation.

    Anti-Angiotensinogen antibody [EPR2931] ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

    Blocking buffer: 1st lanes 1-5: 5% NFDM/TBST; 2nd lanes 1-5: BSA/TBST.

    All lanes: Western blot - Anti-Angiotensinogen antibody [EPR2931] (Anti-Angiotensinogen antibody [EPR2931] ab108334) at 1/1000 dilution

    Lane 1: Mouse kidney whole tissue lysate at 10 µg

    Lane 2: Rat kidney whole tissue lysate at 10 µg

    Lane 3: Human kidney whole tissue lysate at 10 µg

    Lane 4: Mouse liver whole tissue lysate at 10 µg

    Lane 5: Rat liver whole tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/2000 dilution

    Predicted band size: 53 kDa

    Exposure time: 1min

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869), expandable thumbnail

    Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869)

    This data was developed using Anti-Angiotensinogen antibody [EPR2931] ab108334, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: Lane 1: 5% NFDM/TBST. Lane 2: BSA/TBST.

    All lanes: Western blot - Anti-Angiotensinogen antibody [EPR2931] (Anti-Angiotensinogen antibody [EPR2931] ab108334) at 1/1000 dilution

    All lanes: Human fetal liver whole cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG (H+L) peroxidase conjugated at 1/2000 dilution

    Predicted band size: 53 kDa

    Exposure time: 3min

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869), expandable thumbnail

    Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869)

    This data was developed using Anti-Angiotensinogen antibody [EPR2931] ab108334, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Angiotensinogen antibody [EPR2931] (Anti-Angiotensinogen antibody [EPR2931] ab108334) at 1/1000 dilution

    Lane 1: Human fetal artery lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal liver lysate at 10 µg

    Lane 4: HepG2 lysate at 10 µg

    Lane 5: 293T lysate at 10 µg

    Lane 6: Human plasma lysate at 10 µg

    Predicted band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869)

    This data was developed using Anti-Angiotensinogen antibody [EPR2931] ab108334, the same antibody clone in a different buffer formulation.Anti-Angiotensinogen antibody [EPR2931] ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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