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AB215869

Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Angiotensinogen antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SERPINA8, AGT, Angiotensinogen, Serpin A8

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)

This data was developed using ab108334, the same antibody clone in a different buffer formulation.ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)
  • WB

Lab

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)

This data was developed using ab108334, the same antibody clone in a different buffer formulation.

ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

Blocking buffer : 1st lanes 1-5 : 5% NFDM/TBST; 2nd lanes 1-5 : BSA/TBST.

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (<a href='/en-us/products/primary-antibodies/angiotensinogen-antibody-epr2931-ab108334'>ab108334</a>) at 1/1000 dilution

Lane 1:

Mouse kidney whole tissue lysate at 10 µg

Lane 2:

Rat kidney whole tissue lysate at 10 µg

Lane 3:

Human kidney whole tissue lysate at 10 µg

Lane 4:

Mouse liver whole tissue lysate at 10 µg

Lane 5:

Rat liver whole tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/2000 dilution

Predicted band size: 53 kDa

false

Exposure time: 1min

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)
  • WB

Lab

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)

This data was developed using ab108334, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : Lane 1 : 5% NFDM/TBST. Lane 2 : BSA/TBST.

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (<a href='/en-us/products/primary-antibodies/angiotensinogen-antibody-epr2931-ab108334'>ab108334</a>) at 1/1000 dilution

All lanes:

Human fetal liver whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) peroxidase conjugated at 1/2000 dilution

Predicted band size: 53 kDa

false

Exposure time: 3min

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)
  • WB

Unknown

Western blot - Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (AB215869)

This data was developed using ab108334, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Angiotensinogen antibody [EPR2931] (<a href='/en-us/products/primary-antibodies/angiotensinogen-antibody-epr2931-ab108334'>ab108334</a>) at 1/1000 dilution

Lane 1:

Human fetal artery lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Lane 5:

293T lysate at 10 µg

Lane 6:

Human plasma lysate at 10 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2931

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Block with 5% BSA.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p>Block with 5% BSA.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab215869 is the carrier-free version of ab108334.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiotensinogen also known as AGT is a precursor to angiotensin peptides and plays a mechanical role in the renin-angiotensin system. It is a glycoprotein with a mass of approximately 60 kDa and is mainly produced in the liver. This protein circulates in the bloodstream where it waits for renin to cleave it. Angiotensinogen's presence is significant in various tissues but primarily it functions within the plasma after hepatic secretion.
Biological function summary

Angiotensinogen is critical in the regulation of blood pressure and fluid balance. It serves as the substrate for renin an enzyme that initiates a cascade leading to the production of angiotensin I which further converts into active forms like angiotensin II. Angiotensinogen does not operate as part of a larger protein complex but interacts closely with enzymes and other substrates to mediate its effects on the cardiovascular system.

Pathways

Angiotensinogen is an essential component of the renin-angiotensin-aldosterone system (RAAS) which helps regulate blood pressure and electrolyte balance. The pathway involves the conversion of angiotensinogen to angiotensin I by renin followed by the conversion to angiotensin II by angiotensin-converting enzyme (ACE). This system also has connections with proteins such as aldosterone which is involved in sodium and water retention influencing cardiovascular health.

Angiotensinogen links to hypertension and cardiovascular diseases. The protein's interaction with renin and ACE results in the formation of angiotensin II a potent vasoconstrictor that increases blood pressure. Elevated levels of angiotensinogen are often observed in individuals with high 117/85 blood pressure indicating a risk for chronic conditions. Managing angiotensinogen levels through RAAS modulation is a therapeutic strategy for controlling these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.. Angiotensin-2. Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed : 10619573, PubMed : 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed : 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).. Angiotensin-3. Stimulates aldosterone release.. Angiotensin 1-7. Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
See full target information AGT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of pathology & laboratory medicine 147:167-176 PubMed35687787

2022

Plasma Galectin-9 Is a Useful Biomarker for Predicting Renal Function in Patients Undergoing Native Kidney Biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Tsun Tsai,Ruey-Bing Yang,Shuo-Ming Ou,Wei-Cheng Tseng,Kuo-Hua Lee,Chih-Yu Yang,Fu-Pang Chang,Der-Cherng Tarng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com