Anti-Anillin antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (AB5910)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 μg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 μg/ml. DAPI nuclear counterstain was used.

Negative control : Unimmunized goat IgG (10 μg/ml) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 μg/ml).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Anillin antibody (AB5910)

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control : Unimmunized goat IgG (black line) fol

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody (AB5910)

ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.

• ICC/IF

AbReview35161****

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (AB5910)

ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Manoj B Menon