AB99352

Anti-Anillin antibody

Name: Anti-Anillin antibody (ab99352) | Abcam
Brand: Abcam Limited
SKU: AB99352
Price: 545 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Anillin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ANLN aa 1-50.

View Alternative Names

Anillin, ANLN

2 Images

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Immunoprecipitation - Anti-Anillin antibody (AB99352)

Detection of Human Anillin by Western Blot and Immunoprecipitation. Samples : Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99352 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate (lane 1). Anillin was also immunoprecipitated by another rabbit anti-Anillin antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds

All lanes:

Immunoprecipitation - Anti-Anillin antibody (ab99352)

Predicted band size: 124 kDa

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Western blot - Anti-Anillin antibody (AB99352)

All lanes:

Western blot - Anti-Anillin antibody (ab99352) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Predicted band size: 124 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ANLN aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anillin also known as ANLN is a conserved protein with a molecular weight of approximately 124 kDa. It plays an important role in the regulation of the cytoskeleton particularly during cell division. Anillin localizes mainly at the cleavage furrow during cytokinesis and interacts with several cytoskeletal components including actin filaments and myosin. Expression of Anillin occurs in various tissues but it is most abundant in rapidly dividing cells such as those found in cancers and during embryonic development.

Biological function summary

Anillin acts as a scaffold protein coordinating the assembly of the contractile ring during mitosis and cytokinesis. By binding to actin and myosin and sometimes septins Anillin ensures the successful completion of cell division. It forms part of a complex with these proteins promoting stability and functionality of the cleavage furrow. Anillin's structural role is critical for the maintenance and integrity of the dividing cell's structure.

Pathways

Anillin plays a role in the RHO signaling pathway and the cytoskeleton remodeling pathway. It interacts with other proteins in these pathways such as RhoA and Ect2 facilitating the precise control of contractile ring formation. These interactions recruit additional factors to the cleavage site organizing cellular machinery for the final stages of cell division. Anillin's involvement in these pathways underlines its significance in both cell signaling and structural organization.

Anillin has been correlated with cancer progression and metastasis. Overexpression of Anillin has been observed in several types of cancers including breast and lung cancer. It interacts with proteins such as Aurora B kinase linking Anillin to tumor cell proliferation and survival. Understanding Anillin's function and interactions can offer insights into potential therapeutic strategies for targeting dysregulated cell division in cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Required for cytokinesis (PubMed : 16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed : 23870127). May play a significant role in podocyte cell migration (PubMed : 24676636).

See full target information ANLN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40430090

2025

Controlled Exit from the G2/M Checkpoint in RPE-1 Cells Using RO3306: Enrichment of Phase-Specific Cell Populations for In-Depth Analyses of Mitotic Events.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Anglada,Núria Pulido-Artola,Marina Rodriguez-Muñoz,Anna Genesca

Molecular & cellular proteomics : MCP 22:100485 PubMed36549590

2022

Native Size-Exclusion Chromatography-Based Mass Spectrometry Reveals New Components of the Early Heat Shock Protein 90 Inhibition Response Among Limited Global Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul S Samant,Silvia Batista,Mark Larance,Bugra Ozer,Christopher I Milton,Isabell Bludau,Estelle Wu,Laura Biggins,Simon Andrews,Alexia Hervieu,Harvey E Johnston,Bissan Al-Lazikhani,Angus I Lamond,Paul A Clarke,Paul Workman

Cell death and differentiation 30:527-543 PubMed36526897

2022

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fei Cao,Lei Xie,Bei-Bei Tong,Man-Yu Chu,Wen-Qi Shi,Xiang Li,Jian-Zhong He,Shao-Hong Wang,Zhi-Yong Wu,Dan-Xia Deng,Ya-Qi Zheng,Zhi-Mao Li,Xiu-E Xu,Lian-Di Liao,Yin-Wei Cheng,Li-Yan Li,Li-Yan Xu,En-Min Li

Life science alliance 3: PubMed31879279

2019

CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Cansu Uretmen Kagiali,Nazan Saner,Mehmet Akdag,Erdem Sanal,Beste Senem Degirmenci,Gurkan Mollaoglu,Nurhan Ozlu

Nature communications 7:13887 PubMed27976684

2016

Profiling DNA damage response following mitotic perturbations.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni S Pedersen,Gopal Karemore,Thorkell Gudjonsson,Maj-Britt Rask,Beate Neumann,Jean-Karim Hériché,Rainer Pepperkok,Jan Ellenberg,Daniel W Gerlich,Jiri Lukas,Claudia Lukas

PloS one 11:e0154531 PubMed27140416

2016

Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq.

Applications

Species

Unspecified reactive species

S Marthandan,M Baumgart,S Priebe,M Groth,J Schaer,C Kaether,R Guthke,A Cellerino,M Platzer,S Diekmann,P Hemmerich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Anillin antibody

Immunoprecipitation - Anti-Anillin antibody (AB99352)

Western blot - Anti-Anillin antibody (AB99352)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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