AB211868

Anti-Anillin antibody [CL0301]

Name: Anti-Anillin antibody [CL0301] (ab211868) | Abcam
Brand: Abcam Limited
SKU: AB211868
Price: 510 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Anillin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANLN aa 300-500.

View Alternative Names

Anillin, ANLN

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling Anillin with ab211868 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemical analysis of paraffin-embedded Human colorectal tumor tissue labeling Anillin with ab211868 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0301] (AB211868)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0301] (AB211868)

Immunofluorescence analysis of U-251 cells labelling Anilin with ab211868 at 10μg/ml (green). Microtubules are visualised in red.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0301] (AB211868)

Immunofluorescence analysis of A-431 cells labelling Anilin with ab211868 at 10μg/ml (green). Microtubules are visualised in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Anillin with ab211868 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Anillin with ab211868 at 1/50 dilution.

Supplier Data

Western blot - Anti-Anillin antibody [CL0301] (AB211868)

All lanes:

Western blot - Anti-Anillin antibody [CL0301] (ab211868) at 1/500 dilution

All lanes:

U-251 MG cell lysate

Predicted band size: 124 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0301

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANLN aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NQW6

Epitope

Binds to an epitope located within the peptide sequence PSKGELSREICLQSQ as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anillin also known as ANLN is a conserved protein with a molecular weight of approximately 124 kDa. It plays an important role in the regulation of the cytoskeleton particularly during cell division. Anillin localizes mainly at the cleavage furrow during cytokinesis and interacts with several cytoskeletal components including actin filaments and myosin. Expression of Anillin occurs in various tissues but it is most abundant in rapidly dividing cells such as those found in cancers and during embryonic development.

Biological function summary

Anillin acts as a scaffold protein coordinating the assembly of the contractile ring during mitosis and cytokinesis. By binding to actin and myosin and sometimes septins Anillin ensures the successful completion of cell division. It forms part of a complex with these proteins promoting stability and functionality of the cleavage furrow. Anillin's structural role is critical for the maintenance and integrity of the dividing cell's structure.

Pathways

Anillin plays a role in the RHO signaling pathway and the cytoskeleton remodeling pathway. It interacts with other proteins in these pathways such as RhoA and Ect2 facilitating the precise control of contractile ring formation. These interactions recruit additional factors to the cleavage site organizing cellular machinery for the final stages of cell division. Anillin's involvement in these pathways underlines its significance in both cell signaling and structural organization.

Anillin has been correlated with cancer progression and metastasis. Overexpression of Anillin has been observed in several types of cancers including breast and lung cancer. It interacts with proteins such as Aurora B kinase linking Anillin to tumor cell proliferation and survival. Understanding Anillin's function and interactions can offer insights into potential therapeutic strategies for targeting dysregulated cell division in cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for cytokinesis (PubMed : 16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed : 23870127). May play a significant role in podocyte cell migration (PubMed : 24676636).

See full target information ANLN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 4:589-607 PubMed35618940

2022

PARK7/DJ-1 promotes pyruvate dehydrogenase activity and maintains T homeostasis during ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Egle Danileviciute,Ni Zeng,Christophe M Capelle,Nicole Paczia,Mark A Gillespie,Henry Kurniawan,Mohaned Benzarti,Myriam P Merz,Djalil Coowar,Sabrina Fritah,Daniela Maria Vogt Weisenhorn,Gemma Gomez Giro,Melanie Grusdat,Alexandre Baron,Coralie Guerin,Davide G Franchina,Cathy Léonard,Olivia Domingues,Sylvie Delhalle,Wolfgang Wurst,Jonathan D Turner,Jens Christian Schwamborn,Johannes Meiser,Rejko Krüger,Jeff Ranish,Dirk Brenner,Carole L Linster,Rudi Balling,Markus Ollert,Feng Q Hefeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Anillin antibody [CL0301]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0301] (AB211868)

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0301] (AB211868)

Western blot - Anti-Anillin antibody [CL0301] (AB211868)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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