AB211872

Anti-Anillin antibody [CL0303]

Name: Anti-Anillin antibody [CL0303] (ab211872) | Abcam
Brand: Abcam Limited
SKU: AB211872
Price: 510 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(10 Publications)

Mouse Monoclonal Anillin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANLN aa 300-500.

View Alternative Names

Anillin, ANLN

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human testis tissue labeling Anillin with ab211872 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized HeLa cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human tonsil tissue labeling Anillin with ab211872 at 1/50 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U251 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized MCF7 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized A431 cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Negative control : Immunohistochemical analysis of paraffin-embedded, formalin fixed Human liver tissue labeling Anillin with ab211872 at 1/50 dilution shows absence of staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody [CL0303] (AB211872)

Immunofluorescent analysis of formalin fixed, Triton X-100 permealized U2OS cells labeling Anillin with ab211872 at 4 μg/ml dilution showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colorectal cancer tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human stomach tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human colon tissue labeling Anillin with ab211872 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody [CL0303] (AB211872)

Immunohistochemical analysis of paraffin-embedded, formalin fixed Human lung cancer tissue labeling Anillin with ab211872 at 1/50 dilution.

Supplier Data

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-Anillin antibody [CL0303] (ab211872)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Anillin antibody [CL0303] (AB211872)

All lanes:

Western blot - Anti-Anillin antibody [CL0303] (ab211872) at 1/500 dilution

All lanes:

U251 cell line lysate

Predicted band size: 124 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0303

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ANLN aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NQW6

Epitope

Binds to an epitope located within the peptide sequence LRGRFDKGNIWSAEK (438-452 aa) as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Marker for Nucleus without Nucleoli

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anillin also known as ANLN is a conserved protein with a molecular weight of approximately 124 kDa. It plays an important role in the regulation of the cytoskeleton particularly during cell division. Anillin localizes mainly at the cleavage furrow during cytokinesis and interacts with several cytoskeletal components including actin filaments and myosin. Expression of Anillin occurs in various tissues but it is most abundant in rapidly dividing cells such as those found in cancers and during embryonic development.

Biological function summary

Anillin acts as a scaffold protein coordinating the assembly of the contractile ring during mitosis and cytokinesis. By binding to actin and myosin and sometimes septins Anillin ensures the successful completion of cell division. It forms part of a complex with these proteins promoting stability and functionality of the cleavage furrow. Anillin's structural role is critical for the maintenance and integrity of the dividing cell's structure.

Pathways

Anillin plays a role in the RHO signaling pathway and the cytoskeleton remodeling pathway. It interacts with other proteins in these pathways such as RhoA and Ect2 facilitating the precise control of contractile ring formation. These interactions recruit additional factors to the cleavage site organizing cellular machinery for the final stages of cell division. Anillin's involvement in these pathways underlines its significance in both cell signaling and structural organization.

Anillin has been correlated with cancer progression and metastasis. Overexpression of Anillin has been observed in several types of cancers including breast and lung cancer. It interacts with proteins such as Aurora B kinase linking Anillin to tumor cell proliferation and survival. Understanding Anillin's function and interactions can offer insights into potential therapeutic strategies for targeting dysregulated cell division in cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for cytokinesis (PubMed : 16040610). Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis (PubMed : 23870127). May play a significant role in podocyte cell migration (PubMed : 24676636).

See full target information ANLN

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 28: PubMed37539739

2023

Knockdown of ANLN inhibits the progression of lung adenocarcinoma via pyroptosis activation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sheng,Yanhai Kang,Denglin Chen,Linyang Shi

Translational andrology and urology 12:176-186 PubMed36915888

2023

Identification and validation of immunohistochemical marker panels to predict the prognosis of muscle invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yidie Ying,Ziwei Wang,Yuxin Tan,Haotian Cao,Hongliang Gao,Zhensheng Zhang,Shuxiong Zeng,Chuanliang Xu

Molecular therapy : the journal of the American Society of Gene Therapy 30:3477-3498 PubMed35791879

2022

CircRNA Samd4 induces cardiac repair after myocardial infarction by blocking mitochondria-derived ROS output.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zheng,Senlin Huang,Guoquan Wei,Yili Sun,Chuling Li,Xiaoyun Si,Yijin Chen,Zhenquan Tang,Xinzhong Li,Yanmei Chen,Wangjun Liao,Yulin Liao,Jianping Bin

Computational and mathematical methods in medicine 2021:9549287 PubMed34777569

2021

ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Deng,Zhili Xu,Jun Zhou,Ruhu Zhang,Xiaowei Gong

Cell death & disease 12:764 PubMed34344861

2021

Alternatively spliced ANLN isoforms synergistically contribute to the progression of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Erliang Guo,Xionghui Mao,Xueying Wang,Lunhua Guo,Changming An,Cong Zhang,Kaibin Song,Guohui Wang,Chunbin Duan,Xiwei Zhang,Xianguang Yang,Zhennan Yuan,Ji Sun,Xiaomei Li,Weiwei Yang,Hongxue Meng,Susheng Miao

Frontiers in oncology 11:639961 PubMed34295807

2021

Circular RNA circ-MMP11 Contributes to Lapatinib Resistance of Breast Cancer Cells by Regulating the miR-153-3p/ANLN Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Wu,Yi Ren,Rong Yao,Leilei Zhou,Ruihua Fan

Experimental and therapeutic medicine 21:454 PubMed33747188

2021

Actin-binding protein anillin promotes the progression of hepatocellular carcinoma and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huanxia Jia,Zhenya Gao,Fang Yu,Hongfang Guo,Baoyu Li

Journal of clinical laboratory analysis 35:e23635 PubMed33089886

2020

Actin-binding protein Anillin promotes the progression of gastric cancer in vitro and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huanxia Jia,Fang Yu,Baoyu Li,Zhenya Gao

Cancer science 110:3476-3485 PubMed31454442

2019

Identification of new octamer transcription factor 1-target genes upregulated in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shinichiro Yamamoto,Ken-Ichi Takayama,Daisuke Obinata,Kyoko Fujiwara,Daisaku Ashikari,Satoru Takahashi,Satoshi Inoue

Endocrinology 160:463-472 PubMed30649323

2019

Integrative Genomic Analysis of OCT1 Reveals Coordinated Regulation of Androgen Receptor in Advanced Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ken-Ichi Takayama,Yutaka Suzuki,Shinichiro Yamamoto,Daisuke Obinata,Satoru Takahashi,Satoshi Inoue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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