Anti-ANK-3 antibody [2A8]

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-ANK-3 antibody [2A8] (AB315200)

Immunofluorescent analysis of cortex section of rat brain stained with ab315200, dilution 1 : 2,000 in green and costained with rabbit pAb to NF-L, RPCA-NF-L, dilution 1 : 5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The ab315200 antibody stains the axonal initial segments, while the RPCA-NF-L antibody labels dendrites and axons of neuronal cells.

• WB

Supplier Data

Western blot - Anti-ANK-3 antibody [2A8] (AB315200)

Western blot analysis of different tissue and cell lysates using ab315200, dilution 1 : 2,000 in green : [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.

All lanes:

Western blot - Anti-ANK-3 antibody [2A8] (ab315200) at 1/2000 dilution

Lane 2:

NIH-3T3 cell lysate

Lane 3:

HEK293 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

SH-SY5Y cell lysate

Lane 6:

Rat brain tissue lysate

Lane 7:

Mouse brain tissue lysate

Observed band size: 190 kDa

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