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AB213687

Anti-ANK-3 antibody

1

(2 Reviews)

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(1 Publication)

Rabbit Polyclonal ANK-3 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Ankyrin-3, ANK-3, Ankyrin-G, ANK3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse intestine tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANK-3 antibody (AB213687)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 at 1/200 dilution for 40 minutes at 37°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ANK3 aa 1050-1150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q12955

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Alternative Products

Primary Antibodies

AB134317

Anti-ANK-3 antibody [N106/20]

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

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Complementary Products

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB131419

Anti-Ankyrin-B antibody [N105/13]

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Primary Antibodies

AB181593

Anti-Cyclin B1 antibody [EPR17060]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [EPR17060] (AB181593)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.6 Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANK-3 also known as ankyrin-G has a significant role in maintaining the structural integrity and function of cells. It is a cytoskeletal protein with a molecular weight approximately 220 kDa. ANK-3 is expressed in a wide variety of tissues with high expression in the brain heart and skeletal muscle. It links integral membrane proteins to the underlying spectrin-actin cytoskeleton stabilizing the cellular membrane and organizing membrane domains.
Biological function summary

The interaction of ANK-3 with various proteins contributes to cellular activities. ANK-3 binds to key molecules like ion channels and cell adhesion molecules making it an integral part of the cell membrane-cytoskeleton interface. It is a member of the ankyrin family which includes ANK and ANK-106 providing different isoforms like ANK-3 isoforms that contribute to its diverse functional roles. ANK-3 associates with specific complexes facilitating the localization of nodes of Ranvier in axons and maintaining polarized distribution of channels in neurons.

Pathways

ANK-3 serves a major function in the neurobiological and cardiac pathways. It plays a role in the regulation of action potentials in neurons through its interaction with ion channels and is essential in cardiac muscle function by associating with proteins involved in heart rhythm regulation. ANK-3 through these pathways exhibits functional relationships with proteins such as ANK-2 and 2a8 which further influence cellular excitability and signaling.

Disruptions in ANK-3 function associate with neuropsychiatric disorders and cardiac arrhythmias. ANK-3 dysfunction has been implicated in bipolar disorder where genetic variations may affect its molecular interactions leading to altered neuronal signaling. In cardiac contexts improper expression or mutations of ANK-3 can contribute to arrhythmogenic disorders sometimes in coordination with proteins such as n106 and other ankyrins impacting cardiac stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (PubMed : 7836469). In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed : 23903368). Required for intracellular adhesion and junctional conductance in myocytes, potentially via stabilization of GJA1/CX43 protein abundance and promotion of PKP2, GJA1/CX43, and SCN5A/Nav1.5 localization to cell-cell junctions (By similarity).. Isoform 5. May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.
See full target information ANK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:31332 PubMed27510732

2016

Electrical Identification and Selective Microstimulation of Neuronal Compartments Based on Features of Extracellular Action Potentials.

Applications

ICC

Species

Rat

Milos Radivojevic,David Jäckel,Michael Altermatt,Jan Müller,Vijay Viswam,Andreas Hierlemann,Douglas J Bakkum
View all publications
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Product promise

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