Anti-ANK-3 antibody [EPR26551-66] (ab306589)

Rabbit Recombinant Monoclonal ANK-3 antibody. Suitable for IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

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Images

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (AB306589), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Expected	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/300	Notes -
Species Mouse	Dilution info 1/300	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Target data

See full target information ANK3

Function

Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (PubMed:7836469). In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). Required for intracellular adhesion and junctional conductance in myocytes, potentially via stabilization of GJA1/CX43 protein abundance and promotion of PKP2, GJA1/CX43, and SCN5A/Nav1.5 localization to cell-cell junctions (By similarity). Isoform 5. May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.

Alternative names

Ankyrin-3, ANK-3, Ankyrin-G, ANK3

Recommended products

Rabbit Recombinant Monoclonal ANK-3 antibody. Suitable for IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26551-66
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ANK-3 also known as ankyrin-G has a significant role in maintaining the structural integrity and function of cells. It is a cytoskeletal protein with a molecular weight approximately 220 kDa. ANK-3 is expressed in a wide variety of tissues with high expression in the brain heart and skeletal muscle. It links integral membrane proteins to the underlying spectrin-actin cytoskeleton stabilizing the cellular membrane and organizing membrane domains.

Biological function summary

The interaction of ANK-3 with various proteins contributes to cellular activities. ANK-3 binds to key molecules like ion channels and cell adhesion molecules making it an integral part of the cell membrane-cytoskeleton interface. It is a member of the ankyrin family which includes ANK and ANK-106 providing different isoforms like ANK-3 isoforms that contribute to its diverse functional roles. ANK-3 associates with specific complexes facilitating the localization of nodes of Ranvier in axons and maintaining polarized distribution of channels in neurons.

Pathways

ANK-3 serves a major function in the neurobiological and cardiac pathways. It plays a role in the regulation of action potentials in neurons through its interaction with ion channels and is essential in cardiac muscle function by associating with proteins involved in heart rhythm regulation. ANK-3 through these pathways exhibits functional relationships with proteins such as ANK-2 and 2a8 which further influence cellular excitability and signaling.

Associated diseases and disorders

Disruptions in ANK-3 function associate with neuropsychiatric disorders and cardiac arrhythmias. ANK-3 dysfunction has been implicated in bipolar disorder where genetic variations may affect its molecular interactions leading to altered neuronal signaling. In cardiac contexts improper expression or mutations of ANK-3 can contribute to arrhythmogenic disorders sometimes in coordination with proteins such as n106 and other ankyrins impacting cardiac stability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ANK-3 with ab306589 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cerebrum. The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse kidney (PMID: 26727517, 15611082). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat kidney (PMID: 26727517, 15611082). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control: confocal image showing no staining on mouse spleen (PMID: 7615634).

The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: HepG2 (protein atlas database).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: 180 seconds.

Observed bands: 111, 160, 202, 204, 480 kDa.
All lanes: Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589) at 1/1000 dilution
Lane 1: SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 10 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 480 kDa, 204 kDa, 202 kDa, 160 kDa, 111 kDa
Exposure time: 180s
Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lysates of Lanes 3 and 4 were freshly made and used for Western blotting immediately to minimize protein degradation.

The MW is consistent to what has been described in the literature (PMID:7615634).

Negative control: spleen (PMID:7615634).

Exposure time: Lanes 1 and 2: 180 seconds, Lanes 3 and 4: 136 seconds.

Observed MW: 99, 121, 188, 209, 214 kDa.
All lanes: Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589) at 1/1000 dilution
Lanes 1 and 3: Mouse brain tissue lysate 10 μg
Lanes 2 and 4: Mouse spleen tissue lysate 10 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 214 kDa, 209 kDa, 188 kDa, 121 kDa, 99 kDa
Exposure time: 180s
Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 22131424). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cerebrum. The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID: 22131424). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Negative control: No staining on human spleen (PMID: 7615634).

The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lysates of lane 2 was freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time: Lane 1: 26 seconds, Lane 2: 180 seconds.
All lanes: Western blot - Anti-ANK-3 antibody [EPR26551-66] (ab306589) at 1/1000 dilution
Lane 1: Rat brain tissue lysate 10 μg
Lane 2: Rat cerebral cortex tissue lysate 10 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 99 kDa, 188 kDa, 121 kDa, 209 kDa, 214 kDa
Exposure time: 26s
Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] (ab306589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control: confocal image showing no staining on rat spleen.

The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.