Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on human spleen (PMID : 7615634). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ANK-3 with ab306589 at 1/2000 (0.25 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human kidney. The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on rat spleen. The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat kidney (PMID : 26727517, 15611082). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 22131424). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse kidney (PMID : 26727517, 15611082). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat cerebrum. The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse cerebrum. The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC

Supplier Data

Immunohistochemistry - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling ANK-3 with ab306589 at 1/4000 (0.125 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID : 22131424). The section was incubated with ab306589 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling ANK-3 with ab306589 at 1/300 (1.663 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse spleen (PMID : 7615634). The section was incubated with ab306589 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• WB

Supplier Data

Western blot - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : HepG2 (protein atlas database). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 180 seconds. Observed bands : 111, 160, 202, 204, 480 kDa.

All lanes:

Western blot - Anti-ANK-3 antibody [EPR26551-66] (<a href='/en-us/products/primary-antibodies/ank-3-antibody-epr26551-66-ab306589'>ab306589</a>) at 1/1000 dilution

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 μg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 10 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 480 kDa,204 kDa,202 kDa,160 kDa,111 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates of lane 2 was freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : Lane 1 : 26 seconds, Lane 2 : 180 seconds.

All lanes:

Western blot - Anti-ANK-3 antibody [EPR26551-66] (<a href='/en-us/products/primary-antibodies/ank-3-antibody-epr26551-66-ab306589'>ab306589</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate 10 μg

Lane 2:

Rat cerebral cortex tissue lysate 10 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 99 kDa,188 kDa,121 kDa,209 kDa,214 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-ANK-3 antibody [EPR26551-66] - BSA and Azide free (AB306590)

This data was developed using ab306589, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Lysates of Lanes 3 and 4 were freshly made and used for Western blotting immediately to minimize protein degradation. The MW is consistent to what has been described in the literature (PMID : 7615634). Negative control : spleen (PMID : 7615634). Exposure time : Lanes 1 and 2 : 180 seconds, Lanes 3 and 4 : 136 seconds. Observed MW : 99, 121, 188, 209, 214 kDa.

All lanes:

Western blot - Anti-ANK-3 antibody [EPR26551-66] (<a href='/en-us/products/primary-antibodies/ank-3-antibody-epr26551-66-ab306589'>ab306589</a>) at 1/1000 dilution

Lanes 1 and 3:

Mouse brain tissue lysate 10 μg

Lanes 2 and 4:

Mouse spleen tissue lysate 10 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 214 kDa,209 kDa,188 kDa,121 kDa,99 kDa

false

Exposure time: 180s