AB134317

Anti-ANK-3 antibody [N106/20]

Name: Anti-ANK-3 antibody [N106/20] (ab134317) | Abcam
Brand: Abcam Limited
SKU: AB134317
Price: 525 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal ANK-3 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Fusion protein corresponding to ANK3, fused to ANK3.

View Alternative Names

Ankyrin-3, ANK-3, Ankyrin-G, ANK3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

4% formaldehyde-fixed SK-N-BE (human) cells labeling ANK-3 using ab134317 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at rom temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

Immunofluorescent analysis of 4% Formaldehyde-fixed Neuroblastoma cell line (SK-N-BE) labeling ANK-3 with ab134317 at 1/100 dilution. Secondary antibody : Goat Anti-Mouse ATTO 488 at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ankyrin G Antibody. (D) Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling ANK-3 with ab134317 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin G Antibody (D) Composite.

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Western blot - Anti-ANK-3 antibody [N106/20] (AB134317)

Western blot detection of ANK-3 in Rat brain membrane tissues using ab134317 at 1 : 1000 dilution.

All lanes:

Western blot - Anti-ANK-3 antibody [N106/20] (ab134317)

Predicted band size: 480 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N106/20

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Fusion protein corresponding to ANK3, fused to ANK3. The exact immunogen used to generate this antibody is proprietary information.

Q12955

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>1μg/ml of ab134317 is sufficient for detection of Ankyrin G in 20μg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S106-20 to N106/20, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANK-3 also known as ankyrin-G has a significant role in maintaining the structural integrity and function of cells. It is a cytoskeletal protein with a molecular weight approximately 220 kDa. ANK-3 is expressed in a wide variety of tissues with high expression in the brain heart and skeletal muscle. It links integral membrane proteins to the underlying spectrin-actin cytoskeleton stabilizing the cellular membrane and organizing membrane domains.

Biological function summary

The interaction of ANK-3 with various proteins contributes to cellular activities. ANK-3 binds to key molecules like ion channels and cell adhesion molecules making it an integral part of the cell membrane-cytoskeleton interface. It is a member of the ankyrin family which includes ANK and ANK-106 providing different isoforms like ANK-3 isoforms that contribute to its diverse functional roles. ANK-3 associates with specific complexes facilitating the localization of nodes of Ranvier in axons and maintaining polarized distribution of channels in neurons.

Pathways

ANK-3 serves a major function in the neurobiological and cardiac pathways. It plays a role in the regulation of action potentials in neurons through its interaction with ion channels and is essential in cardiac muscle function by associating with proteins involved in heart rhythm regulation. ANK-3 through these pathways exhibits functional relationships with proteins such as ANK-2 and 2a8 which further influence cellular excitability and signaling.

Disruptions in ANK-3 function associate with neuropsychiatric disorders and cardiac arrhythmias. ANK-3 dysfunction has been implicated in bipolar disorder where genetic variations may affect its molecular interactions leading to altered neuronal signaling. In cardiac contexts improper expression or mutations of ANK-3 can contribute to arrhythmogenic disorders sometimes in coordination with proteins such as n106 and other ankyrins impacting cardiac stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (PubMed : 7836469). In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed : 23903368). Required for intracellular adhesion and junctional conductance in myocytes, potentially via stabilization of GJA1/CX43 protein abundance and promotion of PKP2, GJA1/CX43, and SCN5A/Nav1.5 localization to cell-cell junctions (By similarity).. Isoform 5. May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.

See full target information ANK3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 13:1091-1113 PubMed38482441

2024

A comprehensive analysis of the prognostic and immunological role of in pan-cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei Tan,Wei Meng,Zhaosheng Jiang,Ningning Li,Tong Zhang,Jiayi Zhang,Limin Ma,Yangbo Guan

Cancer medicine 10:8673-8692 PubMed34617407

2021

Integrated analysis of patients with KEAP1/NFE2L2/CUL3 mutations in lung adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Jin,Yuansheng Zheng,Zhencong Chen,Fei Wang,Guoshu Bi,Ming Li,Jiaqi Liang,Qihai Sui,Yunyi Bian,Zhengyang Hu,Yulei Qiao,Songtao Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANK-3 antibody [N106/20]

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (AB134317)

Western blot - Anti-ANK-3 antibody [N106/20] (AB134317)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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