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AB169273

Anti-ANKFY1 antibody

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(2 Publications)

Mouse Polyclonal ANKFY1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANKFY1.

View Alternative Names

ANKHZN, KIAA1255, ANKFY1, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, Rab5-binding and ankyrin repeats-containing protein, Rabankyrin-5, Rank-5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ANKFY1 antibody (AB169273)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANKFY1 antibody (AB169273)

Immunofluorescence analysis of HeLa cells, labeling ANKFY1 with ab169273 at 10μg/ml.

Western blot - Anti-ANKFY1 antibody (AB169273)
  • WB

Unknown

Western blot - Anti-ANKFY1 antibody (AB169273)

All lanes:

Western blot - Anti-ANKFY1 antibody (ab169273) at 1 µg/mL

Lane 1:

ANKFY1 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 128 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ANKFY1.

Q9P2R3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKFY1 also known as Ankyrin Repeat and FYVE Domain Containing 1 is a protein with a molecular mass of approximately 152 kDa. It plays a mechanical role in cellular processes by interacting with endosomal membranes through its FYVE domain which binds phosphatidylinositol 3-phosphate (PI3P). ANKFY1 is expressed in various tissues with notable expression in the brain highlighting its significance in neurological functions. The protein structure of ANKFY1 includes ankyrin repeats which facilitate protein-protein interactions and contribute to its function in cellular signaling and membrane trafficking.
Biological function summary

ANKFY1 regulates endosome dynamics and membrane trafficking. It does not usually form part of large protein complexes which allows it to efficiently interact with specific partners. Through its FYVE domain ANKFY1 participates in binding and trafficking of PI3P-enriched vesicles contributing to the maintenance of endosomal pathways. This interaction supports the sorting and distribution of cargo within cells impacting processes like receptor downregulation nutrient transport and signal transduction.

Pathways

Various cellular processes depend on ANKFY1's function in endosomal sorting. It is involved in the phosphatidylinositol signaling pathway affecting how cells interpret various extracellular cues. ANKFY1 also participates in the regulation of the endocytic pathway influencing the internalization and recycling of membrane receptors alongside proteins such as Rab GTPases. These pathways ensure that cellular responses are finely tuned by controlling the localization and activity of various signaling molecules.

Studies link ANKFY1 to certain neurological conditions including Alzheimer's disease. ANKFY1's role in endosomal trafficking can influence amyloid precursor protein (APP) processing thereby impacting the production of amyloid-beta peptides in Alzheimer's pathology. Additionally ANKFY1 is connected to disorders of cognitive function where its interaction with signaling pathways involving synaptic proteins may play a part. These connections highlight the potential importance of ANKFY1 in maintaining neuronal health and function marking it as a point of interest in understanding neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proposed effector of Rab5 (PubMed : 15328530). Binds to phosphatidylinositol 3-phosphate (PI[3]P) (PubMed : 15328530). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of clathrin-coated vesicles with early endosomes (PubMed : 15328530). Involved in macropinocytosis; the function is dependent on Rab5-GTP (PubMed : 15328530). Required for correct endosomal localization (PubMed : 15328530). Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB (PubMed : 24102721). Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner (PubMed : 22284051). Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport (PubMed : 22284051). Required for podocyte migration (PubMed : 29959197).
See full target information ANKFY1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bone & joint research 12:476-485 PubMed37532241

2023

Astragalus polysaccharide promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells by facilitating ANKFY1 expression through miR-760 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfeng Hu,Liu Yang,Yanhua Du,Xiangping Meng,Yuanyuan Shi,Juan Zeng

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 36:272-281 PubMed28574610

2017

Costamere protein expression and tissue composition of rotator cuff muscle after tendon release in sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Severin Ruoss,Christoph B Möhl,Mario C Benn,Brigitte von Rechenberg,Karl Wieser,Dominik C Meyer,Christian Gerber,Martin Flück
View all publications
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Product promise

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