Mouse Polyclonal ANKFY1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANKFY1.
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- Bone & joint research 12:476-4852023Astragalus polysaccharide promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells by facilitating ANKFY1 expression through miR-760 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianfeng Hu,Liu Yang,Yanhua Du,Xiangping Meng,Yuanyuan Shi,Juan ZengPubMed 37532241
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ANKFY1. Database link Q9P2R3
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extent in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Involved in macropinocytosis; the function is dependent on Rab5-GTP. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport.
Alternative names
ANKHZN, KIAA1255, ANKFY1, Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif
Recommended products
Mouse Polyclonal ANKFY1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ANKFY1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ANKFY1. Database link Q9P2R3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ANKFY1 also known as Ankyrin Repeat and FYVE Domain Containing 1 is a protein with a molecular mass of approximately 152 kDa. It plays a mechanical role in cellular processes by interacting with endosomal membranes through its FYVE domain which binds phosphatidylinositol 3-phosphate (PI3P). ANKFY1 is expressed in various tissues with notable expression in the brain highlighting its significance in neurological functions. The protein structure of ANKFY1 includes ankyrin repeats which facilitate protein-protein interactions and contribute to its function in cellular signaling and membrane trafficking.
- Biological function summary
ANKFY1 regulates endosome dynamics and membrane trafficking. It does not usually form part of large protein complexes which allows it to efficiently interact with specific partners. Through its FYVE domain ANKFY1 participates in binding and trafficking of PI3P-enriched vesicles contributing to the maintenance of endosomal pathways. This interaction supports the sorting and distribution of cargo within cells impacting processes like receptor downregulation nutrient transport and signal transduction.
- Pathways
Various cellular processes depend on ANKFY1's function in endosomal sorting. It is involved in the phosphatidylinositol signaling pathway affecting how cells interpret various extracellular cues. ANKFY1 also participates in the regulation of the endocytic pathway influencing the internalization and recycling of membrane receptors alongside proteins such as Rab GTPases. These pathways ensure that cellular responses are finely tuned by controlling the localization and activity of various signaling molecules.
- Associated diseases and disorders
Studies link ANKFY1 to certain neurological conditions including Alzheimer's disease. ANKFY1's role in endosomal trafficking can influence amyloid precursor protein (APP) processing thereby impacting the production of amyloid-beta peptides in Alzheimer's pathology. Additionally ANKFY1 is connected to disorders of cognitive function where its interaction with signaling pathways involving synaptic proteins may play a part. These connections highlight the potential importance of ANKFY1 in maintaining neuronal health and function marking it as a point of interest in understanding neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ANKFY1 antibody (ab169273)
All lanes: Western blot - Anti-ANKFY1 antibody (ab169273) at 1 µg/mL
Lane 1: ANKFY1 transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 128 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ANKFY1 antibody (ab169273)
Immunofluorescence analysis of HeLa cells, labeling ANKFY1 with ab169273 at 10μg/ml.
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