AB211460

Anti-ANKH antibody - C-terminal

Name: Anti-ANKH antibody - C-terminal (ab211460) | Abcam
Brand: Abcam Limited
SKU: AB211460
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ANKH antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANKH aa 450-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA1581, UNQ241/PRO274, ANKH, Mineralization regulator ANKH, ATP carrier protein ANKH, Progressive ankylosis protein homolog, ANK

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKH antibody - C-terminal (AB211460)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKH antibody - C-terminal (AB211460)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling ANKH with ab211460 at 1/10 dilution.

Flow Cytometry (Intracellular) - Anti-ANKH antibody - C-terminal (AB211460)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ANKH antibody - C-terminal (AB211460)

Intracellular flow cytometrical analysis of K562 cells labeling ANKH with ab211460 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-ANKH antibody - C-terminal (AB211460)

All lanes:

Western blot - Anti-ANKH antibody - C-terminal (ab211460) at 1/1000 dilution

All lanes:

Mouse cerebellum tissue lysate at 35 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human ANKH aa 450-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9HCJ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ANKH protein also known as the progressive ankylosis protein homolog has a molecular weight of approximately 53 kDa. ANKH facilitates the transport of inorganic pyrophosphate (PPi) across cell membranes an essential process for mineralization in tissues. It is expressed in a variety of tissues notably in bones and joints. Besides its physiological role ANKH also influences the regulation of PPi levels in extracellular environments which is pivotal for maintaining proper bone and cartilage health.

Biological function summary

ANKH plays a significant role in maintaining PPi homeostasis. It is not known to be a part of any major protein complex but it plays a vital role in skeletal development and mineral metabolism. By managing the balance of PPi ANKH helps inhibit hydroxyapatite crystal formation a critical step in preventing pathological calcification. Its activity contributes to normal joint function and helps maintain cartilage integrity.

Pathways

ANKH is intricately involved in the mineralization pathway and the regulation of phosphate metabolism. It interacts with proteins like osteopontin and ankylosis protein to modulate mineral deposition in the extracellular matrix. The pathway’s focus is on balancing phosphate levels important for bone density and structural integrity. This regulation prevents excessive deposition which can lead to calcification-related conditions.

ANKH mutations are linked to conditions such as chondrocalcinosis and ankylosing spondylitis. These disorders result from impaired PPi transport leading to abnormal calcium pyrophosphate dihydrate crystal deposition in joints. ANKH's interaction with proteins such as osteopontin adds complexity to its involvement in these disorders highlighting its importance in joint health and overall mineral regulation. Proper function of ANKH is therefore essential in sustaining normal physiological bone and joint conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transports adenosine triphosphate (ATP) and possibly other nucleoside triphosphates (NTPs) from cytosol to the extracellular space. Mainly regulates their levels locally in peripheral tissues while playing a minor systemic role. Prevents abnormal ectopic mineralization of the joints by regulating the extracellular levels of the calcification inhibitor inorganic pyrophosphate (PPi), which originates from the conversion of extracellular NTPs to NMPs and PPis by ENPP1 (PubMed : 20943778, PubMed : 32639996, PubMed : 35147247). Regulates the release of the TCA cycle intermediates to the extracellular space, in particular citrate, succinate and malate. Extracellular citrate mostly present in bone tissue is required for osteogenic differentiation of mesenchymal stem cells, stabilization of hydroxyapatite structure and overall bone strength (PubMed : 32639996). The transport mechanism remains to be elucidated (Probable).

See full target information ANKH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33215216

2020

Osteoglycin knockdown promotes vascular smooth muscle cell proliferation and migration in aortic dissection via the VEGF/VEGFR2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zanxin Wang,Xianmian Zhuang,Bailang Chen,Minxin Wei

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ANKH antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKH antibody - C-terminal (AB211460)

Flow Cytometry (Intracellular) - Anti-ANKH antibody - C-terminal (AB211460)

Western blot - Anti-ANKH antibody - C-terminal (AB211460)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise