AB117788

Anti-ANKHD1 antibody

Name: Anti-ANKHD1 antibody (ab117788) | Abcam
Brand: Abcam Limited
SKU: AB117788
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal ANKHD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ANKHD1 aa 1-100.

View Alternative Names

KIAA1085, MASK, VBARP, PP2500, ANKHD1, Ankyrin repeat and KH domain-containing protein 1, HIV-1 Vpr-binding ankyrin repeat protein, Multiple ankyrin repeats single KH domain, hMASK

2 Images

Unknown

Immunoprecipitation - Anti-ANKHD1 antibody (AB117788)

Detection of ANKHD1 by Western Blot of Immunprecipitate.
ab117788 at 1µg/ml staining ANKHD1 in 293T whole cell lysate immunoprecipitated using ab117788 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ANKHD1 antibody (ab117788)

Predicted band size: 269 kDa

false

Unknown

Western blot - Anti-ANKHD1 antibody (AB117788)

All lanes:

Western blot - Anti-ANKHD1 antibody (ab117788) at 0.1 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 50 µg

Predicted band size: 269 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ANKHD1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8IWZ3

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab117788 was affinity purified using an epitope specific to ANKHD1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKHD1 or Ankyrin Repeat and KH Domain Containing 1 is a protein characterized by the presence of ankyrin repeats and KH domains which suggest its role in protein-protein and protein-RNA interactions. The molecular mass of ANKHD1 is approximately 278 kDa. This protein is widely expressed in various tissues with significant presence observed in the brain liver and kidney. Its structural domains facilitate binding which indicates a potential regulatory function in cellular mechanisms.

Biological function summary

ANKHD1 participates in cellular proliferation and differentiation processes. While it is not solely part of a larger protein complex its interaction with other proteins hints at its role in signal transduction pathways. ANKHD1 interacts with molecules involved in these processes influencing gene expression and cell dynamics which are critical for normal cellular functions and adaptation to stimuli. This protein modulates various signaling cascades which can have broad implications within the cell.

Pathways

ANKHD1 interacts with key signaling pathways notably the PI3K/AKT and Wnt pathways. These pathways are essential for cell growth survival and differentiation. In the PI3K/AKT pathway ANKHD1 can affect the phosphorylation state of proteins altering downstream signal cascades. It also associates with proteins such as β-catenin in the Wnt pathway controlling transcriptional activities that influence cell fate and proliferation. The integration in these pathways underlines its significance in maintaining cellular homeostasis and response.

Researchers have linked ANKHD1 to cancer particularly leukemia and breast cancer. Its involvement in proliferative signaling makes it a candidate for oncogenic processes. ANKHD1 overexpression has been observed in cancerous tissues suggesting its potential role in tumorigenesis. In leukemia its interaction with other oncogenes like BCR-ABL may exacerbate disease progression. Similarly its role in cell survival pathways through relations with proteins like AKT highlightd its impact in cancer biology offering potential targets for therapeutic interventions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases.

See full target information ANKHD1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37627542

2023

Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Fosso,Manuela Leo,Livio Muccillo,Vittorio Maria Mandrone,Maria Chiara Di Meo,Annamaria Molinario,Ettore Varricchio,Lina Sabatino

NPJ genomic medicine 7:56 PubMed36171217

2022

Alternative ANKHD1 transcript promotes proliferation and inhibits migration in uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhuizi Sun,Runzhi Huang,Zhenyu Li,Yaru Zhu,Yan Bai,Siyu Wu,Jingshuai Wang,Yan Xiao,Shuyuan Xian,Xiaowen Tong,Jie Zhang,Yi Guo,Yiqin Ouyang

Translational oncology 14:101075 PubMed33773404

2021

SMYD3 confers cisplatin chemoresistance of NSCLC cells in an ANKHD1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Wei Lv,Wen-Qun Xing,Yu-Feng Ba,Hao-Miao Li,Hao-Ran Wang,Yin Li

International journal of oncology 56:1175-1185 PubMed32319569

2020

ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fang Liu,Qiang Han,Xue-Zhu Rong,Man Yang,Yu-Chen Han,Juan-Han Yu,Xu-Yong Lin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANKHD1 antibody

Immunoprecipitation - Anti-ANKHD1 antibody (AB117788)

Western blot - Anti-ANKHD1 antibody (AB117788)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise