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AB222029

Anti-ANKLE1 antibody

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(1 Publication)

Rabbit Polyclonal ANKLE1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANKLE1 aa 350 to C-terminus.

View Alternative Names

ANKRD41, LEM3, ANKLE1, Structure-specific endonuclease ANKLE1, Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, LEM-domain containing protein 3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody (AB222029)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody (AB222029)

Paraffin embedded human bone marrow stained for ANKLE1 using ab222029 at 1/1000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ANKLE1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NAG6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE1 also known as Ankyrin Repeat and LEM Domain containing 1 or LEM3 is a protein that functions as a DNA endonuclease. This protein has a molecular mass of approximately 63 kDa. It is expressed in various tissues with presence reported in the testis ovaries placenta and to a lesser extent in other tissues. ANKLE1 contains ankyrin repeat domains which often mediate protein-protein interactions. Through its mechanical action it is involved in DNA damage response.
Biological function summary

ANKLE1 acts in the maintenance of genomic stability. It plays a role in resolving DNA structures during replication and repair processes. Evidence suggests ANKLE1 does not function as part of a larger protein complex. Instead it performs its endonuclease activity individually to protect cells from DNA damage. This activity makes it essential for proper cell cycle progression and preventing genomic abnormalities.

Pathways

Functional aspects of ANKLE1 integrate into the DNA damage repair pathway specifically the homologous recombination repair pathway. Its endonuclease action helps in processing DNA intermediates that arise during repair. ANKLE1 works in conjunction with other proteins such as RAD51 which facilitates the strand invasion step of homologous recombination. This collaboration helps ensure genomic integrity is preserved during cell division.

Aberrations in ANKLE1 function or expression have associations with certain cancers such as breast cancer. Its role in safeguarding genomic integrity hints at its protective nature against tumorigenesis. Connections between ANKLE1 and proteins like BRCA1 a well-known key player in DNA repair processes illustrate its potential importance in maintaining genomic stability. Studies continue to explore its precise implications in cancer biology and any potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed : 22399800, PubMed : 27245214, PubMed : 32866453, PubMed : 36825683, PubMed : 37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed : 36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed : 36825683).
See full target information ANKLE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JACC. Basic to translational science 9:792-807 PubMed39070274

2024

Myocardial Infarction Suppresses Protein Synthesis and Causes Decoupling of Transcription and Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Shijie Liu,Vaibhav Deshmukh,Fangfei Wang,Jie Liang,Jenna Cusick,Xiao Li,James F Martin
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Product promise

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