AB189990

Anti-ANKLE1 antibody - C-terminal

Name: Anti-ANKLE1 antibody - C-terminal (ab189990) | Abcam
Brand: Abcam Limited
SKU: AB189990
Price: 565 USD
Availability: InStock

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(2 Publications)

Chicken Polyclonal ANKLE1 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANKLE1.

View Alternative Names

ANKRD41, LEM3, ANKLE1, Structure-specific endonuclease ANKLE1, Ankyrin repeat and LEM domain-containing protein 1, Ankyrin repeat domain-containing protein 41, LEM-domain containing protein 3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling ANKLE1 with ab189990 at 5 μg/ml.

Supplier Data

Western blot - Anti-ANKLE1 antibody - C-terminal (AB189990)

Lane 1:

Western blot - Anti-ANKLE1 antibody - C-terminal (ab189990) at 1 µg/mL

Lane 2:

Western blot - Anti-ANKLE1 antibody - C-terminal (ab189990) at 2 µg/mL

All lanes:

293 cell lysate

Predicted band size: 67 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ANKLE1. The exact immunogen used to generate this antibody is proprietary information.

Q8NAG6

Specificity

ab189990 is predicted to not cross-react with ANKLE2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE1 also known as Ankyrin Repeat and LEM Domain containing 1 or LEM3 is a protein that functions as a DNA endonuclease. This protein has a molecular mass of approximately 63 kDa. It is expressed in various tissues with presence reported in the testis ovaries placenta and to a lesser extent in other tissues. ANKLE1 contains ankyrin repeat domains which often mediate protein-protein interactions. Through its mechanical action it is involved in DNA damage response.

Biological function summary

ANKLE1 acts in the maintenance of genomic stability. It plays a role in resolving DNA structures during replication and repair processes. Evidence suggests ANKLE1 does not function as part of a larger protein complex. Instead it performs its endonuclease activity individually to protect cells from DNA damage. This activity makes it essential for proper cell cycle progression and preventing genomic abnormalities.

Pathways

Functional aspects of ANKLE1 integrate into the DNA damage repair pathway specifically the homologous recombination repair pathway. Its endonuclease action helps in processing DNA intermediates that arise during repair. ANKLE1 works in conjunction with other proteins such as RAD51 which facilitates the strand invasion step of homologous recombination. This collaboration helps ensure genomic integrity is preserved during cell division.

Aberrations in ANKLE1 function or expression have associations with certain cancers such as breast cancer. Its role in safeguarding genomic integrity hints at its protective nature against tumorigenesis. Connections between ANKLE1 and proteins like BRCA1 a well-known key player in DNA repair processes illustrate its potential importance in maintaining genomic stability. Studies continue to explore its precise implications in cancer biology and any potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed : 22399800, PubMed : 27245214, PubMed : 32866453, PubMed : 36825683, PubMed : 37216589). Processes chromatin bridges during cytokinesis by cleaving DNA, both priming TREX1-mediated resolution and directly resolving bridges (PubMed : 36825683). Proper processing of chromatin bridges prevents their breakage during cytokinesis, thereby reducing DNA damage, micronuclei formation, and the activation of immune responses such as the cGAS-STING pathway (PubMed : 36825683).

See full target information ANKLE1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:94 PubMed40877951

2025

MS4A6A/Ms4a6d deficiency disrupts neuroprotective microglia functions and promotes inflammation in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Shan Jiao,Yi-Jun Ge,Liang-Yu Huang,Ying Liu,Bang-Sheng Wu,Piao-Piao Lian,Yi-Ning Hao,Shan-Shan Han,Yi-Ting Li,Kai-Min Wu,Chen-Yun Wu,Tian-Lin Cheng,Peng Yuan,Jin-Tai Yu

International journal of cancer 146:3281-3293 PubMed31509622

2019

ANKLE1 N -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Tian,Pingting Ying,Juntao Ke,Ying Zhu,Yang Yang,Yajie Gong,Danyi Zou,Xiating Peng,Nan Yang,Xiaoyang Wang,Shufang Mei,Yuxing Zhang,Changyi Wang,Rong Zhong,Jiang Chang,Xiaoping Miao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANKLE1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE1 antibody - C-terminal (AB189990)

Western blot - Anti-ANKLE1 antibody - C-terminal (AB189990)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise