Rabbit Polyclonal ANKLE2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANKLE2 aa 800-850.
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Publications
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- The Journal of cell biology 218:2136-21492019Repair of nuclear ruptures requires barrier-to-autointegration factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles T Halfmann et. al.PubMed 31147383
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANKLE2 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q86XL3
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed:22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed:22770216). May regulate nuclear localization of VRK1 in non-dividing cells (PubMed:31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed:22770216). Involved in brain development (PubMed:25259927).
Alternative names
KIAA0692, LEM4, ANKLE2, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4
Recommended products
Rabbit Polyclonal ANKLE2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANKLE2 aa 800-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANKLE2 aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q86XL3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ANKLE2 also known as Ankyrin Repeat And LEM Domain Containing 2 is a protein with an approximate mass of 112 kDa. Mechanically ANKLE2 plays a role in regulating nuclear envelope reconstruction during cell division. It is expressed in various tissues including the brain liver and heart. ANKLE2 locates in the nucleus and cytoplasm highlighting its engagement in cellular functions related to the nuclear envelope and mitosis.
- Biological function summary
ANKLE2 contributes to maintaining nuclear integrity and proper cell cycle progression. It does not form part of a known complex but interacts closely with other nuclear envelope proteins. ANKLE2 helps in the disassembly and reassembly of the nuclear envelope supporting chromosome segregation and cell division. Its activity ensures the correct formation of nuclear structures post-mitosis preventing potential errors that could disrupt cellular functions.
- Pathways
ANKLE2 is involved in the mitotic spindle checkpoint pathway and cell cycle control pathway. It interacts with proteins like BAF (Barrier to Autointegration Factor) in these pathways which are essential for nuclear envelope reformation. ANKLE2’s functioning in these pathways demonstrates its participation in monitoring and ensuring successful cell cycle transitions particularly during the mitotic phase.
- Associated diseases and disorders
ANKLE2 is associated with microcephaly and other neurodevelopmental disorders. Mutations in ANKLE2 have connections to impaired brain development highlighting its significance in neural progenitor cell division. ANKLE2 interacts with Zika virus proteins which has been linked to microcephaly resulting from viral infection. Further research into ANKLE2 and related proteins could provide insights into preventing and treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ANKLE2 antibody (ab225905)
All lanes: Western blot - Anti-ANKLE2 antibody (ab225905) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 104 kDa
Exposure time: 30s
Immunoprecipitation - Anti-ANKLE2 antibody (ab225905)
ANKLE2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225905 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225905 at 1 μg/ml.
Lane 1: ab225905 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-ANKLE2 antibody (ab225905)
Predicted band size: 104 kDa
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