AB225905

Anti-ANKLE2 antibody

Name: Anti-ANKLE2 antibody (ab225905) | Abcam
Brand: Abcam Limited
SKU: AB225905
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ANKLE2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANKLE2 aa 800-850.

View Alternative Names

KIAA0692, LEM4, ANKLE2, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4

2 Images

Supplier Data

Immunoprecipitation - Anti-ANKLE2 antibody (AB225905)

ANKLE2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225905 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225905 at 1 μg/ml.

Lane 1 : ab225905 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ANKLE2 antibody (ab225905)

Predicted band size: 104 kDa

false

Supplier Data

Western blot - Anti-ANKLE2 antibody (AB225905)

All lanes:

Western blot - Anti-ANKLE2 antibody (ab225905) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 104 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ANKLE2 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

Q86XL3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225905 was affinity purified using an epitope specific to ANKLE2 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE2 also known as Ankyrin Repeat And LEM Domain Containing 2 is a protein with an approximate mass of 112 kDa. Mechanically ANKLE2 plays a role in regulating nuclear envelope reconstruction during cell division. It is expressed in various tissues including the brain liver and heart. ANKLE2 locates in the nucleus and cytoplasm highlighting its engagement in cellular functions related to the nuclear envelope and mitosis.

Biological function summary

ANKLE2 contributes to maintaining nuclear integrity and proper cell cycle progression. It does not form part of a known complex but interacts closely with other nuclear envelope proteins. ANKLE2 helps in the disassembly and reassembly of the nuclear envelope supporting chromosome segregation and cell division. Its activity ensures the correct formation of nuclear structures post-mitosis preventing potential errors that could disrupt cellular functions.

Pathways

ANKLE2 is involved in the mitotic spindle checkpoint pathway and cell cycle control pathway. It interacts with proteins like BAF (Barrier to Autointegration Factor) in these pathways which are essential for nuclear envelope reformation. ANKLE2’s functioning in these pathways demonstrates its participation in monitoring and ensuring successful cell cycle transitions particularly during the mitotic phase.

ANKLE2 is associated with microcephaly and other neurodevelopmental disorders. Mutations in ANKLE2 have connections to impaired brain development highlighting its significance in neural progenitor cell division. ANKLE2 interacts with Zika virus proteins which has been linked to microcephaly resulting from viral infection. Further research into ANKLE2 and related proteins could provide insights into preventing and treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed : 22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed : 22770216). May regulate nuclear localization of VRK1 in non-dividing cells (PubMed : 31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed : 22770216). Involved in brain development (PubMed : 25259927).

See full target information ANKLE2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:334 PubMed37210384

2023

Klotho prevents transforming growth factor-β2-induced senescent-like morphological changes in the retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Young Jang,Soo-Jin Kim,Kyu-Sang Park,Jeong Hun Kim

The Journal of cell biology 218:2136-2149 PubMed31147383

2019

Repair of nuclear ruptures requires barrier-to-autointegration factor.

Applications

Unspecified application

Species

Unspecified reactive species

Charles T Halfmann,Rhiannon M Sears,Aditya Katiyar,Brook W Busselman,London K Aman,Qiao Zhang,Christopher S O'Bryan,Thomas E Angelini,Tanmay P Lele,Kyle J Roux

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ANKLE2 antibody

Immunoprecipitation - Anti-ANKLE2 antibody (AB225905)

Western blot - Anti-ANKLE2 antibody (AB225905)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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