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AB227538

Anti-ANKLE2 antibody

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(1 Publication)

Rabbit Polyclonal ANKLE2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANKLE2.

View Alternative Names

KIAA0692, LEM4, ANKLE2, Ankyrin repeat and LEM domain-containing protein 2, LEM domain-containing protein 4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE2 antibody (AB227538)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKLE2 antibody (AB227538)

Paraffin-embedded human breast cancer tissue stained for ANKLE2 with ab227538 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ANKLE2 antibody (AB227538)
  • WB

Supplier Data

Western blot - Anti-ANKLE2 antibody (AB227538)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ANKLE2 antibody (ab227538) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ANKLE2. The exact immunogen used to generate this antibody is proprietary information.

Q86XL3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKLE2 also known as Ankyrin Repeat And LEM Domain Containing 2 is a protein with an approximate mass of 112 kDa. Mechanically ANKLE2 plays a role in regulating nuclear envelope reconstruction during cell division. It is expressed in various tissues including the brain liver and heart. ANKLE2 locates in the nucleus and cytoplasm highlighting its engagement in cellular functions related to the nuclear envelope and mitosis.
Biological function summary

ANKLE2 contributes to maintaining nuclear integrity and proper cell cycle progression. It does not form part of a known complex but interacts closely with other nuclear envelope proteins. ANKLE2 helps in the disassembly and reassembly of the nuclear envelope supporting chromosome segregation and cell division. Its activity ensures the correct formation of nuclear structures post-mitosis preventing potential errors that could disrupt cellular functions.

Pathways

ANKLE2 is involved in the mitotic spindle checkpoint pathway and cell cycle control pathway. It interacts with proteins like BAF (Barrier to Autointegration Factor) in these pathways which are essential for nuclear envelope reformation. ANKLE2’s functioning in these pathways demonstrates its participation in monitoring and ensuring successful cell cycle transitions particularly during the mitotic phase.

ANKLE2 is associated with microcephaly and other neurodevelopmental disorders. Mutations in ANKLE2 have connections to impaired brain development highlighting its significance in neural progenitor cell division. ANKLE2 interacts with Zika virus proteins which has been linked to microcephaly resulting from viral infection. Further research into ANKLE2 and related proteins could provide insights into preventing and treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed : 22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed : 22770216). May regulate nuclear localization of VRK1 in non-dividing cells (PubMed : 31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed : 22770216). Involved in brain development (PubMed : 25259927).
See full target information ANKLE2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 71:60-69 PubMed38506035

2024

microRNA-15a-5p suppresses hypoxia-induced tumor growth and chemoresistance in bladder cancer by binding to eIF5A2.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsong Yang,Haoyi Xiang,Mengjing Cheng,Xue Jiang,Ying Chen,Lingyan Zheng,Senxiang Yan,Shufen Zhang,Cheng Zhang,Wei Chen,Dajin Chen
View all publications
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Product promise

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