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AB272894

Anti-ANKRD1 antibody

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(2 Publications)

Rabbit Polyclonal ANKRD1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANKRD1.

View Alternative Names

C193, CARP, HA1A2, ANKRD1, Ankyrin repeat domain-containing protein 1, Cardiac ankyrin repeat protein, Cytokine-inducible gene C-193 protein, Cytokine-inducible nuclear protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ANKRD1 antibody (AB272894)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD1 antibody (AB272894)

A431 (human epidermoid carcinoma cell line) cells stained for ANKRD1 (Green) using ab2728/94 at 1/200 dilution in ICC/IF analysis. Counterstained with Hoechst 33342 (Blue).

Western blot - Anti-ANKRD1 antibody (AB272894)
  • WB

Unknown

Western blot - Anti-ANKRD1 antibody (AB272894)

10% SDS PAGE

All lanes:

Western blot - Anti-ANKRD1 antibody (ab272894) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Predicted band size: 36 kDa

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Western blot - Anti-ANKRD1 antibody (AB272894)
  • WB

Unknown

Western blot - Anti-ANKRD1 antibody (AB272894)

10% SDS PAGE

All lanes:

Western blot - Anti-ANKRD1 antibody (ab272894) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 50 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ANKRD1. The exact immunogen used to generate this antibody is proprietary information.

Q15327

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD1 also known as Ankyrin Repeat Domain 1 or CARP (Cardiac Ankyrin Repeat Protein) is a protein with a molecular weight of approximately 39 kDa. It is involved in cellular mechanics and is widely expressed in heart skeletal muscle and kidney tissues. ANKRD1 functions as a nuclear transcription factor and also binds to other cytoskeletal proteins influencing structural organization and cellular dynamics. Its presence is significant in muscle cells where it participates in responding to mechanical stress.
Biological function summary

ANKRD1 plays a role in the regulation of gene expression especially in the cardiovascular system. It interacts with muscle structural proteins and can form part of a complex with titin which is a giant protein essential for muscle flexibility and elasticity. ANKRD1 modulates muscle development and function by affecting the expression of genes linked to muscle growth and repair. It responds to mechanical stretch in muscles influencing transcriptional activity linked to adaptation and stress resistance.

Pathways

ANKRD1 is involved in the cardiac hypertrophy and muscle cell stress response pathways where its role in these processes is well documented. It interacts with proteins such as p53 contributing to pathways that manage cellular stress and damage response. ANKRD1 participates in signaling events that modify cardiovascular cell growth and survival and this involvement makes it significant in understanding cardiac remodeling and hypertrophic processes.

ANKRD1 is relevant to cardiomyopathy and muscular dystrophy. Abnormal expression or mutations in the ANKRD1 gene are linked to cardiomyopathy a disease where heart muscle becomes thick or weak. It has associations with other muscle regulatory proteins including dystrophin which plays a role in muscle integrity and is implicated in muscular dystrophy. These connections highlight ANKRD1’s importance in both normal cardiac function and in the pathogenesis of heart and muscle diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells.
See full target information ANKRD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 60:101102 PubMed39674114

2024

USP20 mediates malignant phenotypic changes in bladder cancer through direct interactions with YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wensun Chen,Siqi Wu,Yifan Chen,Weijian Li,Yiqing Cao,Yingchun Liang,Xiyu Dai,Xinan Chen,Yilin Chen,Tian Chen,Shenghua Liu,Chen Yang,Haowen Jiang

Journal of breast cancer 24:428-442 PubMed34652079

2021

Long Noncoding-RNA Component of Mitochondrial RNA Processing Endoribonuclease Promotes Carcinogenesis in Triple-Negative Breast Cancer Cells via the Competing Endogenous RNA Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiang Qi,Bo Sun,Beibei Yang,Su Lu
View all publications
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Product promise

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