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AB50852

Anti-ANKRD11 antibody [2022C8a]

4

(2 Reviews)

|

(3 Publications)

Mouse Monoclonal ANKRD11 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ANKRD11 aa 1450-1600.

View Alternative Names

ANCO1, ANKRD11, Ankyrin repeat domain-containing protein 11, Ankyrin repeat-containing cofactor 1

1 Images
Western blot - Anti-ANKRD11 antibody [2022C8a] (AB50852)
  • WB

Unknown

Western blot - Anti-ANKRD11 antibody [2022C8a] (AB50852)

All lanes:

Western blot - Anti-ANKRD11 antibody [2022C8a] (ab50852) at 1/50 dilution

All lanes:

A2058 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody

Predicted band size: 296 kDa

Observed band size: >250 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2022C8a

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ANKRD11 aa 1450-1600. The exact immunogen used to generate this antibody is proprietary information.

Q6UB99

Specificity

This antibody reacts with ANKRD11.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Protein G purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: BSA, 1% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD11 also known by alternate names such as ANKRD13 and KIAA0464 is a mechanistic protein that plays an important part in transcriptional regulation by acting as a co-repressor. The protein has a molecular mass of approximately 285 kDa and features ankyrin repeats which facilitate protein-protein interaction. ANKRD11 is expressed in various tissues with a significant presence in the brain suggesting its importance in neural functions.
Biological function summary

ANKRD11 influences several processes through its involvement in the regulation of gene expression. It is a member of the transcriptional regulatory complex where it interacts with other proteins to modify chromatin structure and influence transcription. ANKRD11 can modulate the activity of several transcription factors impacting cellular proliferation differentiation and other vital cellular functions.

Pathways

ANKRD11 plays a role in key signaling pathways such as the Wnt signaling pathway where it regulates gene expression important for cell fate determination. It interacts with proteins like beta-catenin pivotal in the transduction of Wnt signals. ANKRD11 is also linked with the p53 pathway which governs cell cycle control and apoptosis establishing interfaces with proteins involved in DNA damage response.

ANKRD11 mutations relate to KBG syndrome characterized by developmental delays and craniofacial anomalies. This syndrome showcases the involvement of ANKRD11 in neural development and structural formation. Additionally ANKRD11 dysregulation has been implicated in neurodevelopmental disorders and autism spectrum disorders where it might interact with proteins implicated in synaptic development and neural connectivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin regulator which modulates histone acetylation and gene expression in neural precursor cells (By similarity). May recruit histone deacetylases (HDACs) to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation (PubMed : 15184363). Has a role in proliferation and development of cortical neural precursors (PubMed : 25556659). May also regulate bone homeostasis (By similarity).
See full target information ANKRD11
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37511268

2023

Loss of ANCO1 Expression Regulates Chromatin Accessibility and Drives Progression of Early-Stage Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Yuan,Megan E Barefoot,Kendell Peterson,Moray J Campbell,Jan K Blancato,Manjing Chen,Marcel O Schmidt,Amber J Kiliti,Hong-Bin Fang,Anton Wellstein,Anna T Riegel,Ghada M Sharif

American journal of medical genetics. Part A 182:1690-1696 PubMed32476269

2020

Pathogenic variants in EP300 and ANKRD11 in patients with phenotypes overlapping Cornelia de Lange syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Cucco,Patrizia Sarogni,Sara Rossato,Mirella Alpa,Alessandra Patimo,Ana Latorre,Cinzia Magnani,Beatriz Puisac,Feliciano J Ramos,Juan Pié,Antonio Musio

Molecular endocrinology (Baltimore, Md.) 28:846-59 PubMed24678732

2014

Regulation of HER2 oncogene transcription by a multifunctional coactivator/corepressor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Jason P Garee,Christopher D Chien,Jordan V Li,Anton Wellstein,Anna T Riegel
View all publications
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