JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249036

Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ANKRD2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

ARPP, ANKRD2, Ankyrin repeat domain-containing protein 2, Skeletal muscle ankyrin repeat protein, hArpp

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (AB249036)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (AB249036)

This data was developed using ab151718, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling ANKRD2 with ab151718 at 1/100 diluton.

Western blot - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (AB249036)
  • WB

Unknown

Western blot - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (AB249036)

This data was developed using ab151718, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ANKRD2 antibody [EPR10731(B)] - BSA and Azide free (ab249036) at 1/1000 dilution

All lanes:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10731(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249036 is the carrier-free version of ab151718.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD2 also known as Arpp and MARP2 is an ankyrin repeat protein with a molecular mass of approximately 36 kDa. It is mainly expressed in skeletal muscle and cardiac muscle tissues. The protein functions by interacting with other proteins contributing to muscle structure and function. ANKRD2 binds to titin a critical sarcomeric protein playing a role in muscle elasticity and integrity.
Biological function summary

ANKRD2 acts as a stretch sensor within muscle fibers. This role allows it to be part of a complex responsible for mediating mechanosignaling pathways that respond to mechanical stress or damage in muscle cells. ANKRD2 interacts with other signaling molecules like the transcription factor PML which modulates gene expression in response to mechanical cues.

Pathways

ANKRD2 participates in the muscle-specific mechanotransduction cascade. It plays a significant role in the modulation of MAPK signaling which is important for muscle growth and regeneration. The protein associates with other important proteins in this pathway such as titin and PML which work together to regulate muscle adaptation to physical stress.

ANKRD2 has an association with cardiomyopathy and muscular dystrophy. Its interactions with proteins such as titin and PML may influence the pathology of these diseases as these proteins are key in maintaining muscle and cardiac tissue stability. Disruption in ANKRD2 function or expression can contribute to muscular and cardiac impairments highlighting its importance in muscle-related conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a negative regulator of myocyte differentiation. May interact with both sarcoplasmic structural proteins and nuclear proteins to regulate gene expression during muscle development and in response to muscle stress.
See full target information ANKRD2
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB248814

Anti-ANKRD28 antibody [EPR9211] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-ANKRD28 antibody [EPR9211] - BSA and Azide free (AB248814)

  • 0
  • 0
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB306587

Anti-CXCL10/IP-10 antibody [EPR24674-84]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL10/IP-10 antibody [EPR24674-84] (AB306587)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com