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AB122320

Anti-ANKRD23 antibody

4

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal ANKRD23 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANKRD23 aa 50-200.

View Alternative Names

DARP, ANKRD23, Ankyrin repeat domain-containing protein 23, Diabetes-related ankyrin repeat protein, Muscle ankyrin repeat protein 3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)

Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human prostate with ab122320 at 1/250 dilution showing strong cytoplasmic positivity in smooth muscle cells

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD23 antibody (AB122320)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ANKRD23 antibody (AB122320)

Immunofluorescent staining of human cell line A-431 (Fixation/Permeabilization : PFA/Triton X-100) with ab122320 at 4ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ANKRD23 antibody (AB122320)
Western blot - Anti-ANKRD23 antibody (AB122320)
  • WB

Unknown

Western blot - Anti-ANKRD23 antibody (AB122320)

All lanes:

Western blot - Anti-ANKRD23 antibody (ab122320) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ANKRD23 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q86SG2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD23 also known as Ankyrin Repeat Domain-containing protein 23 is a protein that plays a significant role in cellular processes through mechanical interactions. It weighs approximately 34 kDa. ANKRD23 is expressed in various tissues with a notable presence in the brain suggesting its involvement in neurological functions. This target belongs to the ankyrin repeat protein family recognized for specific protein-protein interactions that aid in maintaining cellular structure and signaling.
Biological function summary

ANKRD23 acts as an adaptor protein facilitating interactions between other proteins and influencing signaling pathways. It is known to be part of protein complexes involved in the regulation of cell cycle and apoptotic processes. Through these functions ANKRD23 supports cellular homeostasis and response mechanisms highlighting its importance in maintaining cellular integrity under different physiological conditions.

Pathways

ANKRD23 is associated with the Wnt signaling pathway and the NF-kB signaling pathway. These pathways are important for regulating gene transcription cellular growth and immune responses. ANKRD23 interacts with proteins such as beta-catenin in the Wnt pathway and RELA in the NF-kB pathway which are instrumental in mediating cellular responses to external stimuli and internal regulatory signals.

ANKRD23 has been linked to neurodegenerative diseases and certain cancers. In the context of neurodegenerative diseases ANKRD23's interactions with proteins such as TAU may influence the progression of conditions like Alzheimer's disease. In cancer altered expression of ANKRD23 could affect the regulatory balance within cell signaling pathways potentially interacting with p53 in cell cycle control and apoptosis evasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in the energy metabolism. Could be a molecular link between myofibrillar stretch-induced signaling pathways and muscle gene expression.
See full target information ANKRD23
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 313:C327-C339 PubMed28615162

2017

Characterization of muscle ankyrin repeat proteins in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan G Wette,Heather K Smith,Graham D Lamb,Robyn M Murphy
View all publications
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Product promise

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