AB86780

Anti-Ankrd26 antibody

Name: Anti-Ankrd26 antibody (ab86780) | Abcam
Brand: Abcam Limited
SKU: AB86780

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(2 Publications)

Rabbit Polyclonal Ankrd26 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ANKRD26 aa 1650 to C-terminus.

View Alternative Names

KIAA1074, ANKRD26, Ankyrin repeat domain-containing protein 26

2 Images

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Immunoprecipitation - Anti-Ankrd26 antibody (AB86780)

HeLa whole cell lysate (1 mg) was immunoprecipitated using 10 μg ab86780 (lane 1), or a control IgG antibody (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab86780 at 1 μg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Ankrd26 antibody (ab86780)

Predicted band size: 196 kDa

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Unknown

Western blot - Anti-Ankrd26 antibody (AB86780)

All lanes:

Western blot - Anti-Ankrd26 antibody (ab86780) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 196 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ANKRD26 aa 1650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPS8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>Or 0.4 - 1μg/ml.</p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ankrd26 also known as Ankyrin Repeat Domain 26 is a protein encoded by the ANKRD26 gene. It has a molecular mass of roughly 216 kDa. Ankrd26 is widely expressed in various tissues with prominent presence in hematopoietic tissues and the central nervous system. Its mechanical function involves interactions with other proteins through its ankyrin repeat domains enabling it to play a role in cellular structural and regulatory processes.

Biological function summary

Ankrd26 acts as a regulatory element involved in signaling pathways that control cell development and proliferation. It is part of protein complexes that regulate cytoskeletal organization impacting cell shape and mobility. This protein has important roles in hematopoietic stem cell function and differentiation influencing the generation of blood cell lineages. Ankrd26's ability to interact with various cellular partners suggests its involvement in broader biological mechanisms.

Pathways

Ankrd26 is implicated in the JAK-STAT signaling pathway and the PI3K-Akt pathway. These pathways oversee cell growth survival and differentiation highlighting Ankrd26's important regulatory nature. Ankrd26 interacts with proteins such as JAK2 and Akt emphasizing its integration into these cellular pathways. This crosstalk allows Ankrd26 to modulate cellular responses to various external signals and maintain cellular homeostasis.

Ankrd26 has associations with thrombocytopenia and certain cancers. Thrombocytopenia relates to mutations in the ANKRD26 gene that impair normal hematopoietic cell function affecting blood platelet levels. Additionally altered expression or mutation of Ankrd26 can contribute to oncogenesis linking it to cancer pathways. Interactions between Ankrd26 and proteins like JAK2 in these contexts underline the importance of this protein in maintaining health and its potential role in disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a regulator of adipogenesis. Involved in the regulation of the feeding behavior.

See full target information ANKRD26

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular and cell biology of lipids 1868:159357 PubMed37315736

2023

Characterization of lipoproteins in human placenta and fetal circulation as well as gestational changes in lipoprotein assembly and secretion in human and mouse placentas.

Applications

Unspecified application

Species

Unspecified reactive species

Titli Nargis,Xinhua Lin,Elena Giordano,Laraib Ijaz,Sarah Suhail,Ellen M Gurzenda,Daniel Kiefer,Loredana Quadro,Nazeeh Hanna,M Mahmood Hussain

The Journal of experimental medicine 218: PubMed33857290

2021

Familial thrombocytopenia due to a complex structural variant resulting in a WAC-ANKRD26 fusion transcript.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Wahlster,Jeffrey M Verboon,Leif S Ludwig,Susan C Black,Wendy Luo,Kopal Garg,Richard A Voit,Ryan L Collins,Kiran Garimella,Maura Costello,Katherine R Chao,Julia K Goodrich,Stephanie P DiTroia,Anne O'Donnell-Luria,Michael E Talkowski,Alan D Michelson,Alan B Cantor,Vijay G Sankaran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ankrd26 antibody

Immunoprecipitation - Anti-Ankrd26 antibody (AB86780)

Western blot - Anti-Ankrd26 antibody (AB86780)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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