JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317195

Anti-ANKRD32 antibody [HL2311]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal SLF1 antibody. Suitable for WB and reacts with Transfected cell lysate samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLF1.

View Alternative Names

ANKRD32, BRCTD1, SMC5-SMC6 complex localization factor protein 1, Ankyrin repeat domain-containing protein 32, BRCT domain-containing protein 1, Smc5/6 localization factor 1

1 Images
Western blot - Anti-ANKRD32 antibody [HL2311] (AB317195)
  • WB

Supplier Data

Western blot - Anti-ANKRD32 antibody [HL2311] (AB317195)

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317195 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ANKRD32 antibody [HL2311] (ab317195) at 1/1000 dilution

Lane 1:

Non-transfected (–) HeLa whole cell extracts at 30 µg

Lane 2:

ANKRD32 shRNA transfected (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2311

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SLF1.

Q9BQI6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ANKRD32 also known as PP2453 or FLJ33840 is an ankyrin repeat domain-containing protein with a mass of approximately 52 kDa. This protein is expressed in various tissues including the heart liver and skeletal muscles. ANKRD32 contains ankyrin repeat motifs that allow it to be involved in protein-protein interactions. These interactions are critical for its mechanical role in cellular processes where it helps in stabilizing other protein complexes.
Biological function summary

ANKRD32 has roles beyond its mechanical functions. It interacts with various cellular components possibly forming complex structures. This interaction suggests its potential involvement in signaling pathways and cellular structural maintenance. ANKRD32 functions appear diversified yet its exact biological roles require further elucidation.

Pathways

ANKRD32 may integrate into key signaling cascades. It is likely associated with the MAPK pathway which controls numerous processes such as cell growth and apoptosis. Additionally ANKRD32 shows potential interaction with components of the NF-kB signaling pathway linked to immune response modulation. Proteins like ERK1/2 involved in the MAPK pathway might engage in functional interaction with ANKRD32.

ANKRD32 holds relevance in certain conditions although specific roles need further investigation. Research suggests links with cardiovascular diseases where protein interaction networks involving ANKRD32 could alter cardiac functions. Moreover studies indicate a potential association with inflammatory disorders likely due to its involvement with NF-kB pathway proteins like RelA. Identifying and understanding these connections could provide insights into disease mechanisms and therapeutic developments.
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed : 25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed : 25931565). Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions (PubMed : 25931565, PubMed : 36373674).
See full target information SLF1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com